한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제22권5호
  • /
  • Pages.459-466
  • /
  • 1994
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Virginiamycin 생합성 유도인자 Virginiae butanolide C에 의한 2차 대사산물 생산의 유도

Induction of Secondary Metabolites by Virginiamycin Inducing Factor, Virginiae Butanolide C

  • 김현수 (계명대학교 자연과학대학 미생물학과) ;
  • 강선영 (계명대학교 자연과학대학 미생물학과)
  • 발행 : 1994.10.01
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초록

Virginiae butanolide C(VB-C) is one of the butyrolactone autoregulators, which triggers the production of virginiamycin in Streptomyces virginiae. Streptomyces longwoodensis was selected as a test strain to investigate new VB-C functions. When 100 ng/ml of the synthetic VB-C was added into the culture at 5 hour and 0 hour, the initial production time of antibiotics and a dark blue pigment were shortened by 4~6 hours and 2~4 hours, respectively. HPLC analysis revealed the production of several new antibiotics by VB-C addition. In the SDS-PAGE analysis of the total protein from mycelium several new protein bands showed up and the amounts of certain protein bands increased in the presense of VB-C. The existence of specific VB-C binding protein was confirmed from S. longwoodensis in relation to VB-C signal transduction. These results suggest that the VB-C might have an ability to induce the production of secondary metabolites in Streptomy- ces longwoodensis.

키워드

참고문헌

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