Cellulose 생성 Acetobacter xylinum KI 균주의 분리 및 특성

Isolation and characterization of Cellulose Producing Acetobacer xylinum KI Strain

  • 차영주 (조선대학교 유전공학과) ;
  • 박경진 (조선대학교 유전공학과) ;
  • 김도경 (조선대학교 유전공학과) ;
  • 전홍성 (조선대학교 유전공학과) ;
  • 이병권 (조선대학교 유전공학과) ;
  • 김근형 (동아전문대학 공업화학과) ;
  • 이숙영 (조선대학교 유전공학과) ;
  • 김성준 (조선대학교 유전공학과)
  • 발행 : 1994.12.01

초록

One strain of cellulose-producing Acetobacter was isolated from the traditionally fermen- ted grape vinegar in Korea. The isolated strain, designated as KI strain was identified as the Acetobacter xylinum with respect to physiological and biochemical characteristics. KI produced acetic acid from ethanol, and then decomposed acetate to CO$_{2}$ and H$_{2}$O. When the isolated strain was cultivated statically in broth culture, a thick cellulose pellicle was formed. KI was tolerance of 8% ethanol and 30% glucose, and the isolate was positive in ketogenesis from glycerol, $\gamma$-pyrone from glucose and fructose, and 2-ketogluconic acid from glucose. KI strain possessed straight-chain C$_{18:1}$, C$_{16:0}$, and C$_{14:0}$ fatty acid, and contained ubiquinone Q$_{9}$ and Q$_{10}$ as isoprenoid quinone. DNA base composition of KI strain was 57.6% G+C.

키워드

참고문헌

  1. Acetic Acid Bacteria Asai,T.
  2. J. Microbiol. Biotech. v.3 Characterization of a new acidophilic Acetobacter sp. strain HA isolated from Korean traditional fermented vinegar Chun,H.S.;S.J.Kim
  3. J. Gen. Mircrobiol. v.21 Cellulose producing by Acetobacter acetigenum and other Acetobacter spp. Dudman,W.F.
  4. J. Bacteriol v.171 Cloning and sequencing of the gene encoding the 72-kilodalton dehydrogenase subunit of alcohol dehydrogenase from Acetobacter aceti Inoue,R.;M.Sunakawa;A.Mori;M.Fukuda
  5. Bergey's Manual of Systematic Bacteriology v.1 Krieg,N.R.;J.G.Holt
  6. J. Biol. Chem. v.265 Identification of the uridine 5'-Diphospho-glucose(UDP-Glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photo affinity probe 5-azido-UDP-Glc Lin,F.C.;R.M.Brown
  7. Biochemical Tests for Identification of Medical Bacteria(2nd ed.) Mac Faddin,J.F.
  8. J. Mol. Biol. v.3 A procedure for the isolation of deoxyribonucleic acid from microorganisms Marmur,J.
  9. Korean J. Biotechnol. Bioeng. v.8 Some properties of Acetoabcter sp. isolated from traditional fermented vinegar Park,J.P.;S.J.Kim;J.C.Ryu;B.S.Pyo;S.W.Kim
  10. J. Gen. Microbiol. v.11 Factors affecting production of cellulose at the air/liquid interface of a culture of A. xylinum Schramm,M.;S.Hestrin
  11. In the Prokaryotes, a Handbook on Habitats, Isolation and Identification of Bacteria The genera Gluconobacter and Acetobacter Swings,J.;J.De Ley
  12. FEMS Microbiol. Lett. v.25 Determination of DNA base composition by reversed-phase high performance liquid chromatography Tamaoka,J.;K.Komagata
  13. Int. J. Syst. Bacteriol. v.36 Acetobacter methanolicus sp. nov., and acidophlilic facultatively methylotrophic bacterium Uhlig,H.;K.Karbaum;A.Steudel
  14. J. Gen. Microbiol. v.128 Cellulose-negative mutant of Acetobacter xylinum Valla,S.;J.Kjosbakken
  15. Appl. and Environ. Microbiol. v.55 Alternative environmental roles for cellulose produced by Acetobacter xylinum Williams,S.;R.E.Cannon
  16. J. Gen. Appl. Microbiol. v.22 Characterization of Acetobacter xylinum by ubiquinone system Yamada,Y.;E.Nakazawa;A.Nozaki
  17. J. Gen. Appl. Microbiol. v.27 The cellular fatty acid composition in acetic acid bacteria Yamada,Y.;M.Nunoda;T.Ishikawa;Y.Tahara