Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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The Synthesis of Ester Compound by Lipase in Organic Solvents

유기용매계에서 리파제에 의한 에스테르 화합물 합성

  • Kim, Boo-Chul (Dept. of Microbiology, Pusan National University) ;
  • Lee, Jae-Dong (Dept. of Microbiology, Pusan National University) ;
  • Lee, Tae-Ho (Dept. of Microbiology, Pusan National University)
  • Published : 1994.06.01
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Abstract

The synthesis of lauryl palmitate from palmitic acid and lauryl alcohol was investigated in organic solvents using lipase. Water-immiscible organic solvent such as hexane, toluenem cyclohexane, and isooctane were found to be suitable of ester synthesis . The effect of water content on the initial rate of conversion was examined . As the content increased, the reaction rate increased. But addition of water in organic solvent decreased therostability of enzyme . The best lauryl palmitate synthesis was achieved with water content of 0.2-0.4% reaction temperature of 4$0^{\circ}C$ and 45$^{\circ}C$ for Candida cylindracea lipase porcine, pancreatic lipase, respectively. when ester synthesis was carried out under the optimum conditions, the conversion yield of palmitate into lauryl palmitate after 70hrs reached 85% and 69 % for the Candida cylindracea lipase and porcine opancreatic lipase, respectivley.

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References

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