고농도 에탄올내성 초산균의 개발 및 배양특성

Investigation of the Cultural Characteristics of High Concentration Ehtanol Resistant Acetobacter sp.

  • 박권삼 (부산수산대학교 식품공학과) ;
  • 장동석 (부산수산대학교 식품공학과) ;
  • 조학래 (동의공업전문대학 식품공업과) ;
  • 박욱연 (부산수산대학교 식품공학과)
  • 발행 : 1994.08.01

초록

식초 생산의 수율을 높이기 위해 재래식 식초생산에 사용되고 있는 종초로 부터 고농도의 에탄올에 내성을 나타내는 균주를 에탄올 순양법으로 개발해 내었다. 에탄올이 5~10% 첨가된 액체배지에 종초를 각각 가하여 에탄올 10%에서 증식이 가능한 균주를 순양 개시 42일만에 분리해 내었고, 이를 동정하여 Acetobactor sp. FM-10이라고 명명하였으며, 이 균주의 배양 특성을 살펴본 결과는 다음과 같다. Acetobactor sp. FM-10의 증식 최적조건을 살펴본 결과, 최적온도는 $30^{\circ}C$였으며, 최적 pH는 5.0이었다. 초산생산에 적합한 배지의 조성은 에탄올 10%, 포도당 5%, yeast extract 1%가 함유된 배지였다. 식초생산 최적조건은 에탄올이 10% 첨가된 배지를 이용하여 $30^{\circ}C$에서 정치배양시 배양 20일만에 최고 산도인 9.0%에 도달하였다.

To increase the yield of acetic acid production, the author developed the bacterial strain which could brow well in high concentration of ehtanol from the seed culture using in conventional vinegar production factory. By attenuation of the isolated strain in the broth media containing 5-10% ethanol, we could get the strain which could grow in the broth medium containing 10% ethanol. This strain was identified and named as Acetobacter sp. FM-10, and it's cultural characteristics were also investigated. The medium containing 10% ethanol, 5% glucose and 1% yest extract was suitable for the acetic acid production with Acetobacter sp. FM-10. Optimum temperature and pH for the growth of Acetobacter sp. FM-10. were $30^{\circ}C$ and 5.0, respectively. The acidity of culture medium was reached to 9.0 % after 20 days static cultivation at $30^{\circ}C$.

키워드

참고문헌

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