Free Amino Acid, SUgar and Enzyme Activity of Honey Harvested in Kangwon Area

강원도산 잡화 벌꿀의 아미노산, 당류 및 효소활성

  • 김복남 (한림전문대학 전통조리과) ;
  • 김택제 (한국과학기술원 화학분석실) ;
  • 최홍식 (부산수산대학교 식품영양학과)
  • Published : 1994.08.01

Abstract

Total nitrogen content, proline, free amino acids, sugars, invertase and diastase activities in native bee-honey (NBH) and foregin bee-honey (FBH) harvested from our different areas of Kangwon , Korea were determined. The total nitrogen contents of NBH and FBH were 0.077$\pm$0.033mg% and 0.055$\pm$0.022mg%, respectively. Proline content in NBH was 42$\pm$10mg% and waqs found to be much lower than that in FHB. Phenylalanine and proline were major free amino acids both in NBH and FBH . The content of total free amino acid in FBH were twice as much as that in NBH , however isomaltose content in NBH were almost two times more than that in FBH. Other sugars including fructose, glucos , sucrose and maltose were also analyzed. No differences were found between NBH and FBH in invertase activities, but comparing to these of FBH ,lower values of diastase activity in NBH were observed.

Keywords

References

  1. J. Assoc. Anal. Chem. v.62 Sugars and sugar products ; Sensitive thin layer chromatographic detection of high fructose corn syrup and other adulterants in honey Kushnir,I.
  2. J. Assoc. Anal. Chem. v.63 Detection of honey adulteration by carbohydrate analysis White,J.W.Jr.
  3. J. Apicultural Research v.6 Composition of honey ; Protein White,J.W.Jr.;Kushnir,I.
  4. J. Apicultural Research v.4 Animo acid analysis of honeys from Eleuen Countries Davies,A.M.C.
  5. J. Assoc. Anal. Chem. v.62 Methods for determining carbohydrates, hydroxymethylfurfural and proline in honey ; Collaborative Study White,J.W.Jr.
  6. J. Assoc. Anal. Chem. v.62 Charcoal column thin layer chromatographic method for high fructose corn syrup and spectrophotometric method for 5-hydroxymethyl-2-furaldehyde in honey ; Collaborative Studies White,J.W.Jr.;Kushnir,I.;Doner,L.W.
  7. J. AOAC v.45 Determination of acidity, nitrogen, and ash in honey White,J.W.Jr.
  8. J. Apicultural Research v.15 Effect of accelerated storage conditions on the chemical composition and properties of Australian honeys ; (1) Colour, acidity and total nitrogen content Wooton,M.;Edwards,R.A.;Gstsjiharemi,R.;Johnson,A.T.
  9. 한국식품과학회지 v.3 각종 식품의 정미 성분에 관한 연구 이성우;김광수;김갑량;이강자;김경희
  10. 강원대학교 연구논문집 v.6 로얄젤리의 free amino acid에 관하여 백광옥;조부연
  11. 한국식품과학회지 v.16 한국산 꿀의 품질 특성 정원철;김만욱;송기준;최언호
  12. 한국식품과학회지 v.17 벌꿀의 조성과 저장중의 diastase 및 hydroxymethylfurfural 함량 변화 한재경;김관;김동윤;김상규
  13. 한국영양식량학회지 v.20 강원도산 잡화 벌꿀의 유기산 및 지방산 특성에 관한 연구 김복남;김택제;최홍식
  14. Official methods of analysis(13th ed.) A.O.A.C.
  15. Advances in Food Research v.24 Honey White,J.W.Jr
  16. Food Technol. v.11 Rapid analysis of carbohydate by high pressure liquid chromatography Conrad,E.C.;Palmer,J.K.
  17. J. Apicultural Research v.17 Proline conten of Unite States honeys White,J.W.Jr.;Rudy,O.N.
  18. Food Technol. Effect of storage and proessing temperatures on honey quality White,J.W.;Tr.Kushnir,I.;Subers,M.H.
  19. New York's Food and Life Science v.8 Detecting honey blended with sugar syrup Shallenberger,R.S.;Guild,W.E.Jr.;Morse,R.A.
  20. Japanese J Food Sci. Technol. v.9 The flavor components of honey Maeda,S.;Mukai,A.;Kosuki,N.;Okada,Y.
  21. J. Food Sci. v.26 Composition of honey ; The effect of stroage on carbohydrates, acidity, and disatase content White,J.W.;Ridthof,M.L.;Kushnir,I.
  22. Acta Chem. Fenn. v.B33 Amino acid in honeys Komamine,A.