The Bleaching Effect of Potato Lipoxygenase Isoenzymes on Chlorophyll a

감자 Lipoxygenase Isoenzymes 의 클로로필 탈색효과

  • 문정원 (부산여자대학교 가정과) ;
  • 조순영 (강릉대학교 식품과학과) ;
  • 서명자 (부산대학교 식품영양학과)
  • Published : 1994.12.01

Abstract

The bleaching effect of chlorophyll a by two lipoxygenase isoenzymes (LOX-1, LOX-2) isolated from potato tuber(variety DEinma ) was studied. In the presence of LOX-1 or LOX0-2 with linoleic acid chlorophyll a bleaching occurred during two isoenzymes-mediated oxidation of linoleic acid. Chlorophyll a bleaching porceeded with decreasing in the formation of conjugated dienes form linoleic acidyb LOX-1 and LOX-2 . In the presence of chlorophyll a, LOX-2 showed a markable decrease inproduction of conjugated dienes from linoleic acid and a higher chlorophyll a bleaching activity. compared with LOX-1. These results suggest chlorophyll-bleaching reaction required intermediates formed during the peroxidation of linoleic acid by lipoxygenase isoenzymes, thus preventing formation of conjugated dienes.

Keywords

References

  1. The enzymes v.8 Lipoxidase Tapple,A.L.;Boyer,P.D.(ed.);Lardy,H.(ed.);Myrback,K.(ed.)
  2. Biochem. Biophys. Acta v.327 Soybean lipoxygenase : An iron-containing dioxygenase Chan,H.
  3. Critical Reviews in Food Science and Nutrition Biochemistry of lipoxygenase in relation to food quality Eskin,N.A.M.
  4. J. Food Sci. v.36 Lipoxygenase content and antioxidant activity of some fruits and vegetables Pinsky,A.;Grossman,S.;Trop,M.
  5. J. Food Sci. v.31 Evaluation of lipid oxidation in plant tissues Rhee,K.S.;Watts,B.W.
  6. Cereal Chem. v.58 Lipoxygenase : Its biochemistry and role in breadmaking Faubion,M.;Hoseney,R.C.
  7. Adv. Chem. Ser. Amer. Chem. Soc. v.136 Food related enzymes Axelrod,B.
  8. Prog. Chem. Fats other Lipids v.15 Plant lipoxygenase Veldink,G.A.;Vliegenthart,J.F.G.;Boldingh,J.
  9. Agr. Biol. Chem. v.34 Soybean lipoxygenase. V. Isolation of lipoxygenase isoenzymes and comparison of their properties Yasumoto,K.;Yamamoto,A.;Mitsuda,H.
  10. Biochem. Biophys. Res. Commun. v.36 On the question of the identity of soybean lipoxygenase and carotene oxidase Kies,M.W.;Haining,J.L.;Pistorius,E.;Schroder,D.H.
  11. Biochim. Biophys. Acta v.327 Purification and properties of a carotene cooxidizing lipoxygenase from peas Arens,D.;Seilmeier,W.;Weber,F.;Kloos,G.;Grosch,W.
  12. J. Agric. Food Chem. v.24 Formation of volatile carbonyl compounds and co-oxidation of β-carotene by lipoxygenase from wheat, potato, flax and beans Grosch,W.;Laskawy,G.;Weber,F.
  13. J. Sci. Food Agric. v.16 Chlorophyll bleaching by legume seeds Holden,M.
  14. Phytochem. v.9 The enzymic breakdown of lipids in potato tuber by phospholipid and galactolipid-acyl hydrolase activities and by lipoxygenase Galliard,T.
  15. Biochem. J. v.124 Lipoxygenase from potato tubers. Partial purification and properties of an enzyme that specifically oxygenates the 9-position of linoleic acid Galliard,T.;Phillips,D.R.
  16. Phytochem. v.12 Lipoxygenase isoenzymes from solanum tuberosum Pinsky,A.;Sporn,J.;Grossman,S.;Rivlin,M.
  17. Plant Cell Physiol. v.15 Metabolism of chlorophyll in higher plants. IV. Relationship of fatty acid oxidation and chlorophyll bleaching in plant extracts Imamura,M.;Shimizu,S.
  18. 한국영양식량학회지 v.22 감자 lipoxygenase isoenzymes의 β-카로텐 탈색효과 문정원;조순영;서명자
  19. Phytochem. v.15 Measurement of lipoxygenase activity in crude and partially purified potatoes extracts Berkeley,H.;Galliard,T.
  20. Protein purification method Ultraviolet spectrophotometry Dunn,M.J.;Harris,E.L.V.(ed.);Angal,S.(ed.)
  21. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry,O.H.;Rosenbrough,N.J.;Farr,A.L.;Randall,R.J.
  22. Phytochem. v.10 Carotene bleaching activities of lipoxygenase and heme proteins as studied by spectrophotometric method Ben-Aziz,A.;Grossman,S.;Ascarelli,I.;Budowski,P.
  23. Biochem. Biophys. Acta v.327 Purification and properties of a carotene co-oxidizing lipoxygenase from peas Arens,D.;Seilmeier,W.;Weber,F.;Kloos,G.;Grosch,W.
  24. J. Biol. Chem. v.134 The coupled oxidation of carotene and fat by carotene oxidase Summer,J.B.;Summer,R.J.
  25. Chemistry and methods of enzymes(3rd) Bleaching bread dough Bohn,R.M.;Haas,L.W.;Summer,J.B.(ed.);Somers,G.F.(ed.)
  26. Fed. Proc. Fed. Am. Soc. Exp. Biol. v.31 Cholesterol peroxidation by soybean lipoxygenase Teng,J.I.;Smith,L.L.
  27. Cereal Chem. v.56 Lipoxygenase and lutein bleaching activity of durum wheat semolina McDonald,C.E.
  28. Food Res. v.17 The loss of chlorophyll in green peas during frozen storage and analysis Wagenknecht,A.C.;Lee,F.A.;Boule,F.P.
  29. Biochemistry of wounded plant tissue Kahl,G.
  30. Ph. D. Dissertation, Purdue University Studies on isoenzymes of soybean lipoxygenase Pistorius,E.
  31. J. Agric. Food Chem. v.27 Some properties of pea lipoxygenase isoenzymes Yoon,S.;Barbara,P.K.
  32. Z. Lebensm Unters. Forsch. v.139 Reaktionswegr zur enzymatischen bildung fluchtiger aldehyde in Erbsen(pisum saivum) Grosch,W.