Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Selection and Cultivation of Microorganism Producing Iron Superoxide Dismutase(Fe-SOD)

Iron Superoxide Dismutase( Fe-SOD)를 생산하는 미생물의 선발 및 배양

  • Published : 1994.12.01
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Abstract

Pseudomonas plycolor was used to investigated the optimal culture condition to examine the various properties of superoxide dismutase (SOD). this SOD was inhibited by $H_2O_2$, azide ion, but not by cyanide ion. This result indicates that the enzyme might be a Fe-SOD. The composition of optimal culture medium for the enzyme production was 3% of glycerin, 1% of polypeptone, 0.5% of meat extract, 0.2% of KCI and the initial ph was 9.0 . The cultivation for the enzyme production was carried out in 500ml shaking flask containing 100ml of the optimal medium at $30^{\circ}C$ on a reciprocal shaker. The enzyme production reached maximum at 15hrs of cultivation and then declined sharply afterward.

비교적 연구가 미비한 Fe-SOD의 효소화학적 특성 및 그 생리적 기능을 검토하기 위해 여러 종의 세균을 대상으로하여 Fe-SOD의 고생산균주를 screening하였다. 그 결과 Fe-SOD를 대량 세포내에 생성하는 Pseudomonas polycolor를 선발하여, 이 균주의 효소생산 최적 배양조건을 설정하였다. 본 균주가 생산하는 효소는 특이적인 저해제의 작용양식에 의해 Fe을 cofactor로 요구하는 Fe-SOD임이 밝혀졌다. SOD 생성을 위한 최적배지조성은 glycerin 3%, polypeptone 1%, meat extract 0.5%, KCI 0.2%이었고, 최적 초발 pH는 9.0이었으며, 이 조건에서 500ml용 shaking flask에 배지 100ml를 넣어 15시간 전 후 배양했을 경우가 효소생산량은 최대가 되었다.

Keywords

References

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