Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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The Effect of Growth Regulators and Light Quality on the Changes in Protein Pattern of Callus from Intergeneric Protoplast Fusion between Nicotiana tabacum and Solanum nigrum

Nicotiana tabacum과 Solanum nigrum의 속간 원형질체 융합에서 유도된 캘러스의 단백질 양태변화에 미치는 생장조절제 및 광선의 효과

  • 김영상 (부산대학교 자연과학대학 생물학과) ;
  • 이동희 (부산대학교 자연과학대학 생물학과)
  • Published : 1994.06.01
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Abstract

The effect of growth regulators (NAA, BA and $ extrm{GA}_3$) and light (blue, red and far-red) on the changes in total protein and thylakoid membrane protein pattern of callus from intergeneric protoplast fusion between Nicotiana tabacum and Solanum nigrw were investigated. When the callus were irradiated with different wavelengths of light, blue and red light accelerated the synthesis of total proteins and thylakoid membrane proteins. Particularly, red light led to an increase in the protein synthesis compared to blue light. When the callus were subjected to various combinations of growth regulators, NAA+$ extrm{GA}_3$ and NAA+BA treatments induced remarkable increase of total proteins and thylakoid membrane proteins accumulation, particularly in the combination of NAA+$ extrm{GA}_3$. NAA.$ extrm{GA}_3$ treatment with irradiation of red ligh showed highest value in the accumulation of total proteins and thylakoid membrane proteins. We conclude that simultaneous application of red light and NAA+$ extrm{GA}_3$ treatment may induce synergistic effect in the synthesis of total proteins and thylakoid membrane Proteins.

담배(Nicotiana tabacum)와 까마중(Solanum migrum)의 솩간 원형질체 융합으로 유도된 켈러스를 재료로하여 캘러스의 전체 단백젤과 틸라코이드막 단백질의 양태변화를 중심으로 식물생장 조절물질과 단색광의 생리적 상호효과를 조사하였다. 여러 단색 광선을 캘러스에 조사하였을때, 적색 및 청색광이 캘러스의 전체 단백질과 킬라코이드막 단백질의 합성을 촉진하였으며 , NAA+$ extrm{GA}_3$ 와 NAA+BA의 조합구에서 캘러스의 전체 단백질과 틸라코이드막 댄백질의 축척이 활발히 일어났으며, NAA+$ extrm{GA}_3$처리구에서 더욱 효과적 이였다. NAA+$ extrm{GA}_3$ 처리구에 청색광, 적색광 및 근적외광을 제각각 처리하였을때 캘러스의 전체 단백질과 킬라코이드막 단백질의 합성은 적색광에 의하여 가장 촉진되었다. 따라서 적색광과 NAA+$ extrm{GA}_3$구의 동시처리가 캘러스의 전체 단백질 및 킬라코이드막 단백질의 합성을 상승적으로 촉진함을 보였다.

Keywords

References

  1. Eur. J. Biochem. v.118 Biosynthesis of the light-harvesting chlorophyll a/b protein: Polypeptide turnover in darkness Bennet,J.
  2. Planta v.152 Subculture-induced protein synthesis in tissue culltures of Glycine max and Phaseolous vulgaris Bevan,M.;D.H.Northcote
  3. Z. Pflanzenphysiol. v.104 Plant regeneration from mesophyll protoplasts of eggplant Bhatt,D.P.;G.Fassuliotis
  4. Planta v.158 Regulation of swelling of etiolated-wheat leaf protoplats by phytochrome and gibbrelic acid Blakeley,S.S.;B.Thomas;J.L.Hall;D.Vince-Prue
  5. Plan cell Rep. v.10 Molecular analysis of the nuclear organellar genotype of somatic hybrid plants between tomato (Lycopersicon esculenturn) and Lycopersicon chilense Bonnema,A.B.;J.M.O`Connell
  6. Plant Physiol. v.75 Auxin conjugation by tobacco mesophyll protoplasts Caboche,M.;G.Aranda;A.M.Poll;J.C.Heut;J.J.Leguay
  7. Plant Physiol. v.90 Galatolipids of thylakoid pigment protein complexes separated electrophoretically from thylakoids of Dunaliella salina labeled with radioactive fatty acids Cho,S.H.;G.A.Thompson
  8. Plant Cell Physiol. v.29 no.5 Swelling of etiolated oat protoplasts induced by phytochrome, cAMP and gibberellic acid: A kinetic study Chung,C.H.;S.U.Lim;P.S.Song;J.D.Berlin;J.R.Goodin
  9. Plants Proteolytic Enzymes v.Ⅱ Chloroplast senescence and proteolytic ezymes Dalling,M.J.;A.M.Nettleton;M.J.Dalling(ed.)
  10. Theor. Appl. Genet. v.84 Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents: Nucleus-chloroplast interaction Derks,F.H.M.;J.C.Hakkert;W.H.J.Verbeek;C.M.Colijin-Hooymans
  11. Plant Cell. Tissue and Organ Culture v.8 Effect of synthetic auxins on callus induction from tea stem tissue Frisch,C.H.;N.D.Camper
  12. Photochem. Photobiol. v.45 Photoreceptor interaction in plant photomorphogenesis: The limits of experimental techniques and their interactions Gaba,V.;M.Black
  13. Plant Physiol v.85 Phytochrome regulation of the response to exogenous gibberellins by epicotyls of Vigna sinensis Garcia-Martinez,J.L.;B.Keith;B.A.Bonner;A.Stafford;L.Rappaport
  14. Plant Physiol. v.63 Comparison of the molecular weights of proteins synthesized by isolated chloroplasts with those which appear during greening in Zea mays Grebanier,A.E.;K.E.Steinback;L.Bogorad
  15. Planta v.126 Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media Kao,K.N.;M.R.Michayluk
  16. Plant Cell Physiol. v.27 Effect of phytochrome on the uptake of gibberellin by protoplasts of Nicotiana glutinosa L. Keppler,L.D.;D.Mertz
  17. Korean J. Bot. v.33 no.4 Induction of callus and culture of protoplasts from cotyledon-originated callus in Alnus Hirsuta Kim,H.H.;C.S.An
  18. Plant Cell Physiol. v.27 no.2 Swelling of etiolated oat protoplasts induced by cAMP and red light Kim,Y.S.;D.K.Moon;J.R.Goodin;P.S.Song
  19. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T₄ Laemmli,U.K.
  20. Biochem. Biophys. Acta v.723 Changes in composition and function of thylakoid membranes as a result of photosynthetic adaptation of chloroplasts from pea plants grown under different light conditions Leong,T.Y.;J.M.Anderson
  21. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;N.J.Rosebrough;A.Lewis Farr;R.J.Randall
  22. Progress in Plant Growth Regulation Phytochrome modulation of gibberellin metabolism in cowpea epicotyls Martinez-Garcia, J. F.;J. L. Garcia-Martinez;C.M.Karssen(ed.);L. C. Van Loon (ed.);D.Vreugdenhil(ed.)
  23. Plant Physiol. v.85 Regulation of light-harvesting chlorophyll protein biosynthesis in greening seedling Mathis,J.M.;K.O.Burkey
  24. Plant Physiol. v.81 Accumulation of chlorophyll, chloroplastic proteins, and thylakoid membranes during reversion of chromoplasts to chloroplasts in Citrus sinensis epicarp Mayfield,S.P.;A.Huff
  25. Encyclopedia of Plant Physiology NS v.16A An introduction to photomorphogenesis for the general reader Morh,H.;W.Shrosphire
  26. Plant Cell Physiol. v.30 no.3 Supramolecular assembly and function of subunits associated with the chlorophyll a-b light-harvesting complex Ⅱ(LHCⅡ) in soybean chloroplasts Morrissey,P.J.;R.E.Glick;A.Melis
  27. Biochem. Biophys. Acta. v.864 The molecular organization of the photosynthetic membranes of higher plants Murphy,D.J.
  28. Plant Physiol. v.93 Distribution of chlorophyll-protein complexes during chilling in the light compared with heat-induced modifications Ovaska,J.;P.Maenpaa;A.Nurmi;E.M.Aro
  29. Plant Cell Culture Isolation, culture and genetic manipulation of plant protoplasts Power,J.B.;J.V.Chapman;R.A.Dixon(ed.)
  30. Plant Physiol v.91 Rapid effects of red light on the isopentenyladenosine content in Scots pine seeds Qamaruddin,M.;E.Tillberg
  31. Mol. Gen. Genet. v.236 Fertile asymmetric somatic hybrids between Lycopersicon esculentum Mill. and Lycopersicon peruvianum var. dentanum Dun. Ratushnyak,Y.I.;N.N.Cherep;A.V.Zavgorodnyaya;S.A.Latypov;I.V.Borozenko;R.I.Rachkovskaya;Y.Y.Gleba
  32. Plant Science Letters v.34 Shoot regeneration from hormoneantonomes callus from shoot cultures of several sugarbeet (Beta vulgaris L.) genotypes Sauders,J.W.;M.E.Daub
  33. Mol. Gen. Genet. v.242 Asymmetric fusion between protoplasts of tomato (Lycopersicon esculentum Mill.) and gamma-irradiated protoplasts of potato (Solanum tuberosum L.) : the effects of gamma irradiation Schoenmakers,H.C.H.;J. J. M. van der Meulen-Muisers;M.Koorneef
  34. Theor. Appl. Genet. v.60 Chloroplast DNA assorts randomly in intraspecific somatic hybrids of Nicotiana debneyi Scowcroft,W.R.;P.J.Larkin
  35. Plant Physiol. v.59 Biochemical responses of pea root tissue to cytokinin Shininger,T.L.;L.D.Polly
  36. Proc. Natl. Acad. Sci. USA. v.81 Indole-3-acetic acid sensitization of phytochrome-controlled growth of coleoptile sections Shinkle,J.R.;W.R.Briggs
  37. Biochim. Biophys. Acta v.1183 Redox titration of multiple protein phosphorylations in pea chloroplast thylakoids Silverstein,T.;L.Cheng;J.F.Allen
  38. Plant cell physiol. v.23 Physical and hormonal factors influencing organogenesis in Rudbeckia bicolor explants cultured in vitro Tanimoto,S.;H.Harada
  39. Jour. of Exp. Bot. v.38 Hormonal control of organogenesis and somatic embryogenesis in Beta vulgaris callus Tetu,T.;R.S.Sangwan;B.S.Sangwan-Norrel
  40. Eur. J. Biochem. v.41 Composition of the photosynthetic apparatus of normal barley leaves and a mutant lacking chlorophyll b Thornber,J.P.;H.Highkin
  41. Plant Physiol. v.61 Red light-inhibited mesocotyl elongation in maize seedlings Vanderhoef,L.N.;W.R.Briggs
  42. Plant Cell Reports v.4 Suspension culture of sugarbeet (Beta vulgaris L.) : Induction and habituation of dedifferentiated and self regenerating cell lines Van-Geyt, L. N.;M.Jacobs
  43. Plant Physiol. v.77 An analysis of growth regulator interactions and gene expression during auxin-induced cell elongation using cloned complementary RNAs Walker,J.C.;J.Legocka;L.Edelman;J.L.Key
  44. Mol. Gen. Genet. v.241 Analysis of nuclear and organella DNA of somatic hybrid calli and plants between Lycopersicon spp. and NIcotianoa spp. Wolters,A.A.;A.C.Vergunst;F.van der Werff;M.Koorneef
  45. Plant Physiol. v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murashige,T.;F.Skoog