Diazinon이의 계배 기형 유발에 미치는 영향 1. 계배형태와 콜린성 봉쇄약물과의 관계

Teratogenic Effects of Diazinon in Chick Embryos 1. Effects of Diazinon Treatment on Morphology and Cholinergic Blocking Agents

Teratogenic effects of diazinon were assessed morphologically and cholinergic blocking agents. Diazinon at doses ranging from 25 to 2000 ug /egg, was Injected on day 3 of incubation. TD50s were different for the various teratogenic signs (wry neck, micromelia, abnormal feathering, abnormal beak and curled claws). The threshould dose for wry neck was higher than threshould dose for other signs； 40 ug/egg produced substantial micromelia, abnormal feathering. abnormal beak and curled claws, but gave no signs of wry neck. In contrast to the teratogenic doses, the LD50 of diazinon was very high (above 2000 ug /egg). One of the characteristics of diazinon-induced teratogenesis was reduced body weight (78.7％) and body length (73.8%). Maximal teratogenic effects, scored as signs of retarded growth, wry neck micromelia, abnormal feathering, abnormal beak, and curled claws, were produced when the insectcide was administered on the third or fourth day. The threshold dose for type II teratogenic signs(such as wry neck and short neck) was higher than for type I (such as micromelia and abnormal feathering). Morphological studies, using atropine and gallamine, suggested that nicotine but not muscarinic receptors may be involved in the mechanism of diazinon induced type II malformations.