신속한 BOD 측정장치의 개발과 응용

Development of Assembly for Fast BOD Estimation and Its Applications

Trichosporon cutaneum을 투석막과 기체투과막 사이에 고정화시켜 산소전극에 부착함으로써 연속적으로 측정할 수 있는 BOD 센서를 개발하였다. 이 센서의 최적 조건은 pH 7.0, $32{\sim}33^{\circ}C$의 온도, $2{\sim}3ml/min$의 flow rate 및 0.1M phosphate 완충용액이었다. ${\Delta}DO$에 대하여 GGA 표준용액의 농도를 도시하였을 때 GGA 60mg/l 이하에서 직선성을 나타니었으며, 30mg/L의 GGA를 사용하였을 때 재현성은 3%의 오차범위를 나타내었다. DO값은 30일 동안 거의 일정하였으며, 감응시간과 회복시간은 각각 약 5분과 10분이었다. 이 센서를 이용하여 실제 폐수의 BOD를 측정하여 $BOD_5$법과 비하였다.

A microbial BOD sensor for the continuous estimation of BOD was been developed by immobilizing Trichosporon cutaneum, which was immobilized between a dialysis and a gas-permeable membrane, on an oxygen electrode. The optimum pH and temperature for BOD measurement using this sensor were pH 7.0 and $32{\sim}33^{\circ}C$, respectively. The best result was obtained at 2~3ml/min flow rate in 0.1M phosphate buffer solution. A linear relationship was observed between ${\Delta}DO$ and the concentration of standard GGA solution below 60mg/l(90ppm $BOD_5$). The reproducibility was found to be within 3% for the standard solution containing glucose 30mg/l and glutamic acid 30mg/l. The output DO value of this sensor was almost constant for 30 dalys. The response time and the recovery time were about 5 and 10 min, respectively. This sensor was employed for the BOD measurement of waste pollutants and was compared with $BOD_5$ method.