Clinical and Experimental Reproductive Medicine
- Volume 21 Issue 2
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- Pages.207-214
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- 1994
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
A Study on the Methodology of Chromosome Preparation from Blood Culture
혈액세포를 이용한 염색체 분리 분석에 관한 방법적 고찰
- Sohn, S.H. (Department of Dairy Science & Technology, Chinju National University) ;
- Chung, K.M. (Hankook Institute of Life Science)
- Published : 1994.08.31
Abstract
This study was carried out to develop the methodology of chromosome preparation from blood cultures in mammals which included human, mouse, cattle and pig. For karyotyping, 0.5-5.0ml of peripheral blood were collected and cultured. The satisfactory results were obtained from macroculture and microculture in all species. In culture, the patterns of cell growth were no difference among media except serum concentration and mitogen supplement. The presence of mitogen and fetal bovine serum in medium significantly affected the mitotic index. The optimal culture condition was 37