The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Standardization of Chemically Defined Medium for the Production of Mycelium and Basidiocarps in Flammulina velutipes

팽나무버섯 균사체 및 자실체 생산을 위한 화학합성배지의 최적화

  • 송치현 (대구대학교 생물공학과) ;
  • 이창호 (대구대학교 생물공학과) ;
  • 안장혁 (고려대학교 식품공학과) ;
  • 홍범식 (고려대학교 식품생명공학과) ;
  • 양한철 (고려대학교 식품공학과)
  • Published : 1995.03.30
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Abstract

Nutritional requirements for the growth of Flammulina velutipes were studied. Mannitol, glutamic acid and ammonium nitrate were chosen for the maximum mycelial growth when various carbon and nitrogen sources tested. Optimum C : N ratio for the mycelial growth was 20 : 1. Potassium dihydrogen phosphate was selected among the phosphate sources. Magnesium sulphate and thiamine HCl stimulate mycelial growth. Final compositions of optimized chemically defined medium were 1.5% mannitol, 0.082% $NH_4NO_3$, 0.312% glutamic acid, 0.25% $KH_2PO_4$, 0.06% $MgSO_4{\cdot}7H_2O\;and\;0.3\;{\mu}g/l$ thiamin HCl. This medium not only support mycelial growth but also induce fruit body formation.

Flammulina velutipes(팽나무버섯)의 영양요구성에 대한 연구로써 각종 탄소원 및 질소원을 처리한 결과 mannitol과 glutamic acid 그리고 ammonium nitrate가 최대 균사체 생장을 보였으며, 최적 C : N ratio 로써 20 : 1이 선정되었다. Phosphate source로는 potassium dihydrogen phosphate가 좋은 결과를 나타내었으며, magnesium sulphate와 thiamin HCl이 균사체 생장을 촉진하였다. 이상의 결과로 최적 화학합성배지의 조성은 1.5% mannitol, 0.082% $NH_4NO_3$, 0.312% glutamic acid, 0.25% $KH_2PO_4$, 0.06% $MgSO_4{\cdot}7H_2O$ 그리고 $0.3\;{\mu}g/l$ thiamin HCl로 나타났다. 이와 같은 배지의 조성은 균사체 생장 뿐만 아니라 자실체 생장에도 효과가 있는 것으로 나타났다.

Keywords

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