The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Biodegradation of Poly (3-hydroxybutyrate) by Penicillium pinophilum

Penicillium pinophilum에 의한 Poly (3-hydroxybutyrate)의 생분해

  • Kim, Mal-Nam (Department of Biology, Sangmyung Women's University) ;
  • Kang, Eun-Jung (Department of Biology, Sangmyung Women's University)
  • 김말남 (상명여자대학교 생물학과) ;
  • 강우정 (상명여자대학교 생물학과)
  • Published : 1995.12.30
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Abstract

Biodegradability of poly (3-hydroxybutyrate) (PHB) by Penicillium pinophilum was investigated by the modified Sturm Test. The biodegradability measurement by this method was more reproducible than other conventional activated sludge methods. Optimum inoculum size for the PHB biodegradation was 1% (v/v). The degradation appeared to occur not only on the sample surface but also inside the sample because the biodegradation did not increase quite proportionally with the sample surface area. The biodegradation rate increased to an asymptotic value as the nitrogen content in the test medium increased, indicating the nitrogen source was needed for the synthesis of the PHB depolymerase.

Poly (3-hydroxybutyrate)(PHB) 의 Penicillium pinophilum에 의한 생분해성을 변형 Sturm Test 법으로 조사하였다. 활성오니를 사용한 경우보다 PHB의 생분해성이 비교적 재현성있게 측정되었으며 PHB의 생분해를 가장 빠르게 진행시키는 최적 포자 접균량은 1 %(v/v)이었다. 생분해 속도는 시료의 표면적에 따라 증가하였으나 비례적으로 상승하지 않아 분해가 시료의 표면 뿐만 아니라 내부에서도 진행됨을 나타내었다. 반응 배지내의 질소원 함량에 따라 PHB의 생분해 속도는 증가하다가 점근값을 보여 질소원이 depolymerase 효소의 합성에 필요한 원소임을 보였다.

Keywords

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