Effects of Rehydration Methods on viability after Freeze-drying of Streptomyces aureofaciens

Streptomyces aureofaciens 동결건조후 재수화 방법이 생존도에 미치는 영향

  • Lee, Hyean-Woo (Department of Microbiological Engineering, Kon-Kuk University) ;
  • Yi, Dong-Heui (Department of Microbiological Engineering, Kon-Kuk University) ;
  • Lee, No-Woon (Department of Microbiological Engineering, Kon-Kuk University)
  • 이현우 (건국대학교 미생물공학과) ;
  • 이동희 (건국대학교 미생물공학과) ;
  • 이노운 (건국대학교 미생물공학과)
  • Published : 1995.12.31

Abstract

In order to examine the effect of rehydration methods on viability after freeze-drying of Streptomyces aureofaciens, we labeled the DNA of S. aureofaciens with $^3H-adenine$. Extracellular radioactivity levels appeared to be high in the rehydrated solutions after freeze-drying than freezing-thawing. In effects of rehydration after freeze-drying, the viability of the cell appeared about 20% in case of with admitting air at single ampule, but that of which appeared about 91% in case of without admitting air at double ampule. Thus, S. aureofaciens cells were damaged during the process of rehydration after freeze-drying.