Surface Activities of Ginseng Saponins and Their Interactions with Biomolecules'(V) Ginseng Saponins Can Be Vsed in Cytochrome c Isolation

인삼사포닌의 계면활성 및 생체 분자와의 작용 : (V) 미토콘드리아 Cytochrome c 단리에서 인삼사포닌의 추출효과

The effects of treating bovine heart mitochondria with potassium chloride and surfactants such as digitonin and n-dodecy-$\beta$-maltoside (DMS) including plant saponins on extracting cytochrome c were examined. The spectra given by the cytochrome c-containing solutions from the extraction were inspected to ascertain whether ginseng and bellflower saponins could be used instead of the generally- employed surfactants of digitonin and DMS. These studies implied that the effect of ginseng saponins is superior to that of digitonin but inferior to that of DMS, and give rise to the idea of substitutional property of ginseng saponins for the widely-employed surfactants in the extraction of mitochondria intermembrane cytochrome c. The substitution for the solubilizing surfactants by bellflower saponins could, however, not presumably be anticipated; while ginseng saponin mixture are a suitable substitute.