Archives of Pharmacal Research
- Volume 18 Issue 6
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- Pages.454-457
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- 1995
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
A Modified Alkaline Hydrolysis of Total Ginsenosides Yielding Genuine Aglycones nad Prosapogenols
- Im, kwang-Sik (College of Pharmacy, Pusan National University) ;
- Chang, Eun-Ha (College of Pharmacy, Pusan National University) ;
- Je, Nam-Gyung (Institute of Life Science Research, Choseon Pharm and Trading)
- Published : 1995.12.01
Abstract
To improve the yield of genuine aglycones from glycosides, the conditions of alkaline hydrolysis were investigated, and a modified method was established. The modified method empolyed pyridine as an aprotic solvent. To complete the hydrolysis and obtain 20(S)-protopanaxadiol (1) and 20(S)-protopanaxatriol(2), which are the genuine aglycones of ginsenosides, total ginsenosides were refluxed with sodium methoxide in pyridine. Addition of methanol, a protic polar solvent to the reaction miuxture, led partial hydrolysis yielding a mixture of the genuine prosapogenols. Of the prosapogenols compound 3 and 6 characteristically possessed D-glucopyranosyl moiety attached at the sterically hindered C-20 hydroxyl group. 3 and 6 were not obtaijned by other hydrolysisw methods except by the soil bacterial hydrolysis.
Keywords
- Modified alkaline hydrolysis;
- Total ginsenoside;
- 20(S)-protopanaxadiol;
- 20(S)-pro-topaxatrol;
- Genuine aglycones;
- Genuine prosapogenols;
- Aprotic solvent