Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Levels of the Proline and Glycine Betaine Transport Systems of Staphylococcus aureus at High Osmolarity

고농도 삼투환경에서 생성되는 포도상구균의 Proline과 Glycine Betaine Transport System의 수준변화에 관한 연구

  • 배지현 (계명대학교 가정대학 식생활학과)
  • Published : 1995.08.01
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Abstract

Staphylococcus aureus, the most salt-tolerant nonhalophilic bacterium, is the only foodborne pathogen that is able to grow at a levels below 0.90. The fundamental osmorgulatory strategy used by this organism involves the accumulation of intracellular compatible solutes such as proline or glycine betaine which are accumulated by transport and act as osmoregulators in cells. In this study, levels of proline transport systems and glycine betaine transport system of S. aureus were examined when cells are grown at high osmolarity. The levels of all three transport systems within S. aureus were elevated at high osmolarity and the most dramatic increase was found for the low-affinity proline transport system. However, in 5mM glycine betaine-supplemented medium, the level of the low-affinity proline transport system did not become elevated when cultures were grown at high osmolarity. The metabolic fate of the accumulated proline and glycine getaine was investigated by thin-layer chromatography an found to be not metabolized by S. aureus.

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References

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