흰쥐에 초산납투여가 신장조직중 Free Radical 대사효소 활성과 초미형태학적 변화에 미치는 영향

Administration of Lead Acetate on the Activity of Free Radical Meta-bolizing Enzyme and Ultrastructural Changes in Rat Kidney

  • 김승필 (계명대학교 자연과학대학 공중보건학과) ;
  • 윤종국 (계명대학교 자연과학대학 공중보건학과) ;
  • 박관규 (계명대학교 의과대학 병리학교실)
  • 발행 : 1995.06.01

초록

To investigate the ultrastructural changes of kidney and clarify to a cause of its changes in lead intoxicated rats, the 0.5% lead acetate administed orally to the rats and those were sacrifled at 2 day, 1, 2, 4, 6 and 8 week after the treatment of lead acetate. Each extirpated kidney was histopathologically examined under the electron microscopy and histochemical examination was also carried out. Concomitantly, the activity of free radical metabolizing enzyme was determined. The blood levels of lead concentration showed a gradual increase from the first group reaching the plateau at the one or two week group with the slightly decreasing value throughout the whole course of the experiment. And the urinary ALA concentration showed a gradual increase from the first group to the 8 week group. In the kidney tissue of rat sacrified at 6 week, the proximal tubular cells showed dilatation of endoplasmic reticulum, mitochondrial swelling, increased numbers of secondary lysosomes and myelin figure-like residual bodies on electron microscope and oxygen free radicals are identified by histochemistry on light microscope whereas there were no differences in the activity of catalase and glutathione peroxidase between the lead acetate treated group and control group. But the activity of xanthine oxidase was more increased in lead acetate treated rats than control group. Furthermore, the superoxide dismutase activity was significantly increased in the experimental group than the control group. In conclusion, it is assumed the kidney damage in lead intoxicated rat may be induced by free radicals.

키워드

참고문헌

  1. Mtehod of Enzymatic Analysis v.2 Catalase Aebi,H.;H.V.Bergme yer(ed.)
  2. Merck index (ed 11) Budavarie,S.
  3. Endothelial superoxide production in bupper perfused rat lungs, demonstrated by a new histochemical technique v.65 no.4 Methods in laboratory investigation Charles,F.B.;Meloney,D.C.;John,J.T.;Stephen,F.B.;William,A.H.
  4. Robbins pathologic basis of disease (ed. 4) Cotran,R.S.;Kumar,V.;Robbins,S.L.
  5. Robbins pathologic basis of diseases(4th ed.) Cotran,R.S.;Kumar,V.;Robbins,S.L.
  6. Nephron v.2 Les 1 sions r nales du Saturnisme humain et exp rimental Galle,P.;Morel-Maroger,L.
  7. Arch Environ Health v.20 Lead dosage and the role of the intranuclear inclusion body. An experimental study Goyer,R.A.;Leonard,D.L.;Moore,J.F.;Rhyne,B.C.;Krigman,M.R.
  8. Lab Invest v.22 Lead and protein content of isolated intranuclear inclusion bodies from kidneys of lead poisoned rats Goyer,R.A.;May,P.;Cates,M.Krigman,M.R.
  9. Pathology of the kidney(ed. 4) Heptinstall,R.H.
  10. Are free radicals involved in lead poisoning Xenobiotica v.21 Hermes-Lima,M.;Pereica,B.;Bechara,E.J.
  11. Biochem Biophysic Acta v.1056 Damage to rat liver mitochondria promoted by delta-aminolevulinic acid-generated reactive oxygen species: Connections with acute intermittent porphyria and lead-poisoning Hermes-Lima,M.;Valle,V.G.;Vercesi,A.E.;Bechara,E.J.
  12. Anderson's pathology (ed. 8) Kissane,J.M.
  13. J. Biophysic Biochem Cytol v.9 Improvement in epoxy resin embedding method Luft,J.H.
  14. Arch Biochem Biophys v.255 no.2 Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation Martin,J.P.;Dailey,M.;Sugarman,E.
  15. Br J Exp Pathol. v.69 Localization of lead in the kidney and liver of rats treated in vivo with lead acetate: Ultrastructural studies on unstained sections Matteo,A.R.;Shiv.C.K.;George,D.V.
  16. J. Lab Clin Med. v.70 Studie, on the quantitative and qualitative characterization of erythrocytes glutathion peroxidase Paglia,E.A.;Valentine,W.N.
  17. J Cell Biol. v.17 The use of lead citrate at high pH as an electron opaque stain in electron microscopy Reynolds,E.S.
  18. Analytical methods in toxicology Stahr,H.M.
  19. J Biol Chem v.244 no.14 The regulation of rat liver xanthine oxidase Stirpe,F.;Della Corte,E.
  20. Br J Ind Med v.26 A simple method for the quantitative analysis of urinary delta-aminolevulinic acid to evaluate lead absorption Wada,O.;Toyokawa,K.;Urata,G.;Yano,Y.;Nakao,K.
  21. J Lab Clin Med v.94 Alterations of intestinal and renal functions in rats after intraperitoneal injections of lead acetate Wapnir,R.A.;Moak,S.A.;Lifshitz,F.;Teichberg,S.
  22. J Biophysic Biochem Cytol v.6 Staining of tissue sections for electron microscopy with heavy metals Watson,M.L.
  23. Chem Pharm Bull v.25 no.3 Origin of plasma uric acid induced by 1-ep-inephirine Yonetani,Y.;Ishii,M.;Yamada,K.;Ogawa,Y.
  24. Keimyung Research Journal v.2 A modified colorimetric assay for xanthine oxidase in rat liver extracts Yoon,C.G.
  25. Chem Pharm Bull v.25 Epinephrine-induced hyperuricemia in experimental animals Yonetani,Y.;Douzaki,T.;Ogawa,Y.