Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Characteristics of ustilago maydis virus of SH14 killer strain isolated in Korea

  • Hwang, Seon-Hee (Department of Microbiology, Kangwon National University) ;
  • Jung, Cheong-Hwan (Department of Microbiology, Kangwon National University) ;
  • Yie, Se-Won (Department of Microbiology, Kangwon National University)
  • Published : 1995.06.01
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Abstract

SH-14, a novel killer strain of Ustilago maydis was isolated in Korea. It has been reported in other papers that the toxin specificity and double-stranded RNA pattern of SH-14 strain were different from other laboratory strains. In this paper, we analyzed the biochemical characteristics of U. maydis SH-14 virus. Three distinctive peaks were isolated from CsCl density gradient, designated as top (T), intermediate (I) and bottom (B) components. We found that the densities of each components, 1.285, 1.408 g/cm$\^$3/, respectively, are very similar to those of other strains. As previously reported by the analysis of dsRNA in each component, the dsRNA segments are separately encapsidated. Capsid protein of SH-14 virus consists of two proteins about 70 Kd shown by SDS-PAGE analysis. Electron microscopic examination of the virus particles revealed that UmV particles are very similar in size and morphology to all isolates as well as all lab-strains. In order to test immunological cross reactivity of UmV, werstern bolt analysis was carriedout with antiserum against A8 virus. All capsid protein had positive reaction against A8 antibody which indicated that UmV are immunologically cross-reactive with all isolates from Korea. The results presented in this paper may show that UmV isolated from SH-14 strain has very similar biochemical characteristics to those of other UmV. However, the difference in the toxin specificity and the molecular weight of toxin protein from the SH-14 strain has us to conclude that U. maydis SH-14 strain is a new killer type.

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References

  1. Mol. Cell. Biol. v.4 RNA-polymerase activity in virions from Ustilago maysid Ben-Zvi, B.S.;Y.Koltin;M.Mervarrch;A. Ta-markin
  2. Virol. v.113 The molecular weight and packaging of dsRNA in the mycovirus from Ustilago maydis Killer strain Bozarth,R.F.;Y.Koltin;M.B.Weissman;R.L.Parker;R.F.Dalton;R.Steinlauf
  3. Inter. Rev. Cytol. v.117 RNA plasmids Brown,G.G.;P.M.Finnegan
  4. Virus Res. v.3 In vitro translation of the major capsid polypeptide from Ustilago maydis Dalton,R.E.;G.K.Podila;W.H.Flurkey;R.F.Bozarth
  5. Mycologia v.73 Fungal virus populations in corn smut from Connecticut Day,P.R.
  6. Mol. Gen. Genet. v.233 Immunity and resistance to the KP6 toxin of Ustilago maydis Finkler,A.;T.Peery;J.Tao;J.A.Bruenn;Y.Koltin
  7. J. Biol. Chem. v.267 Interaction of two cis-sites with the RNA replicase of the yeast L-A Fusimura,T.;R.B.Wickner
  8. Biochem. Biophys. Res. Comm. v.162 Ustilago maydis virus P4 killer toxin:Charcterization, partial amino terminus sequence, and evidence for glycosylation Ganesa,C.;W.H.Flurkey;Z.I.Randhawa;R.F.Bozarth
  9. Kor. J. Mocrobiol. v.31 Genomic variation and toxin specificity of Ustilago maydis virus isolated in Korea Hwang,S.H.;S.W.Yie
  10. Mol. Microbiol. v.11 Structure and heterologous expression of Ustilago maydis viral toxin KP4 Park,C.M.;J.A.Bruenn;C.Ganesa;W.H.Flurkey;R.F.Bozarth;Y.Koltin
  11. Mol. Cell. Biol. v.7 Virus-encoded toxin of Ustilago maydis:Two Polypeptides are essential for activity Peery,T.;T. Shabat-Brand;R.Steinlauf;Y.Koltin;J.A.Bruenn
  12. J. Gen. Virol. v.68 Identification and comparison of viral genes coding for capsid proteins of Ustilago maydis virus Podila,G.K.;W.H.Flurkey;R.F.Bozarth
  13. Genetics v.60 Compatibility reactions on solid medium and interstrain inhibition in Ustilago maydis Puhalla,J.F.
  14. Proc. Natl. Acad. Sci. v.89 RNA-dependent RNA Polymerase concensus sequence of the L-A double-stranded RNA virus:Definition of essential domains Ribas,J.C.;R.B.Wickner
  15. Yeast v.8 Genetic analysis of maintenance and expression of L and M double-stranded RNAs of yeast killer virus K28 Schmitt,M.J.;D.J.Tipper
  16. Mycological guidebook Stevens,R.B.
  17. Mol. Cell. Biol. v.10 Ustilago maydis KP6 killer toxin:Structure, expression in Saccharomyces cerevisiae, and relationship to other cellular toxins Tao,J.;I.Ginsberg;N.Banerjee;W.Held;Y.Koltin;J.A.Bruenn
  18. J. Virol. v.68 Ribosome frameshifting requires a pseudoknot in the Saccharomyces cerevisiae double-stranded RNA virus Tzeng,T.H.;C.L.Tu;J.A.Bruenn
  19. Ann. Rev. Microbiol. v.46 Double-Stranded and single-stranded RNA virus of Saccharomyces cerevisiae Wickner,R.B.
  20. Phytophath v.63 Heterokaryon transfer of virus-like particles associated with a cytoplasmically inherited determinant in Ustilago maydis Wood,H.A.;R.F.Bozarth
  21. Virus Res. v.12 Semi-conservative strand-displacement transcription of the M2 dsRNA segment of Ustilago maydis virus Yie,S.W.;G.K.Podila;R.F.Bozarth
  22. Kor. J. Microbiol. v.30 Molecular biological characterization of Ustilago maydis virus isolated in Korea Yie,S.W.;H.T.Choi