Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhanced stability of Pseudomonas sp. Endo-1,4-$\beta$$\beta$-1,4-Glucosidase Gene

Pseudomonas sp. 유래 Endo-1,4-$\beta$-Glucanase 및$\beta$-1,4-Glucosidase 유전자의 안정성 개선

  • 김양우 (경상대학교 식품공학과) ;
  • 전성식 (경상대학교 식품공학과) ;
  • 정영철 (진주전문대학 식품영양과) ;
  • 노종수 (동의공업전문대학 환경공업과) ;
  • 성낙계 (경상대학교 식품공학과)
  • Published : 1995.12.01
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Abstract

To improve stability of recombinant DNA pLC1 encoding endoglucanase gene and pGL1 encoding $\beta $-glucosidase gene, DNA fragments of genes coding endoglucanase and $\beta $-glucosidase were cloned within the recA gene on a pDR1453, and the pDRE10 and pDRG20 of recombinant plasmids were integrated into the recA gene on the E. coli 1100 chromosomal DNAs. The stability of inheritance was completely maintained in E. coli 1100; Transformants E. coli 1100/pDREIO and pDRG20 were expressed well by recA promoter and increased endoglucanase and $\beta $-glucosidase activities. This method can be used as a model to improve the stability of recombinant plasmid in large scale culture.

Keywords

References

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