Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular cloning and sequence Analysis of the Gene for SecY from Streptomyces coelicolor (Muller)

Streptomyces coelicolor에서 secY 유전자의 클로닝과 염기서열 결정

  • 김상숙 (명지대학교 생명과학과, 생명과학연구소) ;
  • 현창구 (명지대학교 생명과학과, 생명과학연구소) ;
  • 김영민 (연세대학교 이과대학 생물학과, 생물산업소재연구센터) ;
  • 이주헌 (연세대학교 이과대학 생물학과, 생물산업소재연구센터) ;
  • 정인권 (연세대학교 이과대학 생물학과, 생물산업소재연구센터) ;
  • 김대명 (청주대학교 유전공학과) ;
  • 서주원 (명지대학교 생명과학과, 생명과학연구소)
  • Published : 1995.12.01
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Abstract

SecY is a central component of the protein export machinery that mediate the translocation of secretory proteins across the plasma membrane of Escherichia coli. In order to study the mechanism of protein secretion in Streptomyces, we have done cloning and sequencing of the Streptomyces coelicolor secY gene by using polymerase chain reaction method. The nucleotide sequence of the gene for SecY from S. coelicolor showed over 58% identity to that of M. luteus. The deduced amino acid sequences were highly homologous to those of other known SecY polypeptides, all having the potential to form 10 transmembrane segments, and especially second, fifth, and tenth segments were particularly conserved, sharing greater than 75% identity with W. lute s SecY. We propose that the conserved membrane-spanning segments actively participate in protein export. In B. subtilis and E. coli, the secY gene is a part of the spc operon, is preceded by the gene coding for ribosomal protein L15, and is likety coupled transcriptionally and translationally to the upstream L15 gene. In the other hand, secY gene of S. coelicolor and M. luteus have its own promoter region, are coupled translationally with adk gene and pr sented in adk operon.

Keywords

References

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