Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Analysis of Lambda Site-specific Recombination Inermediates Generated by Synthetic Substrates

합성 기질에 의해 형성된 Lambda Site-specific Recombination 중간 대사물의 분석

  • 이나영 (연세대학교 공과대학 식품생물공학과 및 생물산업소재연구센터) ;
  • 유승구 (연세대학교 공과대학 식품생물공학과 및 생물산업소재연구센터)
  • Published : 1995.06.01
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Abstract

Integrase (Int) carries out the cutting and resealing of attachment (att) site DNA via a covalent Int-DNA intermediate. A family of synthetic substrate DNAs was designed to accumulate Int-DNA intermediate. Int-DNA intermediates accumulated by half substrate was analyzed by SDS- KCI precipitation and restriction digestion. The results showed that Int-half DNA intermediate was circular and contained covalently bound Int molecule. Int-DNA intermediates were also trapped with three other kinds of synthetic substrates.

Keywords

References

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