Korean Journal Plant Pathology (한국식물병리학회지)

The Korean Society of Plant Pathology (한국식물병리학회)
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Nucleotide Sequence Analysis of Movement Protein Gene from Tobacco Mosaic Virus Korean Pepper (TMV-KP) Strain

담배 모자이크 바이러스 한국고추계통에서 분리한 이동 단백질 유전자의 염기서열 분석

  • 이재열 (경북대학교 자연과학대학 미생물학과) ;
  • 정동수 (경북대학교 자연과학대학 미생물학과) ;
  • 장무웅 (영남대학교 이과대학 생물학과) ;
  • 최장경 (강원대학교 농과대학 농생물학과)
  • Published : 1995.02.01
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Abstract

Complementary DNA of the movement protein (MP) gene of tobacco mosaic virus Korean pepper strain (TMV-KP) was synthesized from purified TMV-KP RNA by using the reverse transcription and polymerase chain reaction (PCR) system. The synthesized double stranded cDNA was cloned into the plasmid pUC9 and transformed into Escherichia coli JM110. The movement protein gene of TMV-KP of the selected clones was subjected to sequence analysis by Sanger's dideoxy chain termination method. The complete sequence of viral MP gene from TMV-KP strain was 807 nucleotides long. The nucleotide of MP gene from TMV-KP has thirteen and two nucleotide differences from TMV vulgarae (TMV-OM) and Korean (TMV-K) strains, respectively. Thus, the nucleotide sequence of TMV-KP MP gene showed higher homology of 99% with that of TMV-K MP gene.

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