Cultural Characterization of Bacteriolytic Bacillus subtilis SH-1 Isolated from Pusan Coastal Sea

해양에서 분리한 용균세균인 Bacillus subtilis SH-1의 배양특성

  • 류병호 (경성대학교 식품공학과) ;
  • 진성현 (부산광역시 보건환경연구원)
  • Published : 1995.12.01


Bacillus subtilis SH-1 have been isolated and identified from coastal sea, in Pusan, The optimal cultural characterization of Bacillus subtilis SH-1 for 속 production of bacteriolytic enzyme was determained. Bacillus subtilis SH-1 produced the bacteriolytic enzyme well in the medium consist of 1.0% glucose, 1.0% yeast extract, 1.0% NaCI, 0.02% $K_2HPO_4,\;0.002%\;MgSo_4{\cdot}7H_2O,\;0.001%\;MnSO_4{\cdot}5H_2O,\;0.0001%\;FeSO_4{\cdot}7H_2O$. The optimal medium pH, incubation temperature, and shaking tome for the highest production of the enzyme were 8.0, $30^{\circ}C$ and 28 hours respectively.