Cultural Characterization of Bacteriolytic Bacillus subtilis SH-1 Isolated from Pusan Coastal Sea

해양에서 분리한 용균세균인 Bacillus subtilis SH-1의 배양특성

Bacillus subtilis SH-1 have been isolated and identified from coastal sea, in Pusan, The optimal cultural characterization of Bacillus subtilis SH-1 for 속 production of bacteriolytic enzyme was determained. Bacillus subtilis SH-1 produced the bacteriolytic enzyme well in the medium consist of 1.0% glucose, 1.0% yeast extract, 1.0% NaCI, 0.02% $K_2HPO_4,\;0.002%\;MgSo_4{\cdot}7H_2O,\;0.001%\;MnSO_4{\cdot}5H_2O,\;0.0001%\;FeSO_4{\cdot}7H_2O$. The optimal medium pH, incubation temperature, and shaking tome for the highest production of the enzyme were 8.0, $30^{\circ}C$ and 28 hours respectively.

해양에서 분리한 용균활성이 우수한 균주인 Bacillus subtilis SH-1의 배양특성을 조사하여 다음과 같은 결과를 얻었다. Bacillus subtilis SH-1에 의한 용균효소 생산을 위한 치적조건인 1.0% glucose, 1.0% yeast extract, 1.0% NaCl, 0.02% $K_2HPO_4,\;0.002%\;MgSo_4{\cdot}7H_2O,\;0.001%\;MnSO_4{\cdot}5H_2O,\;0.0001%\;FeSO_4{\cdot}7H_2O\;(ph\;8.0)$의 배지로 $30^{\circ}C$에서 진탕배양하여 배양 28시간일 때 효소활성이 가장 높았다.