Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Chinese Radish Juice as a Growth Substrate for the Production of Candida utilis ATCC 42416 Biomass

무즙을 이용한 Candida utilis ATCC 42416균체 생산

  • Published : 1995.06.01
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Abstract

Chinese radish juice(CRJ) was used as a culture medium for the production of Candida utilis ATCC 42416 biomass. Soluble solid and total sugar contents of Chinese radishes were in the range between 5.5 and $8.8^{\circ}$Brix and 3.5 and 6.5%, respectively. Since sugar in radishes are in readily usable forms, pretreatm ent procedures were not necessary after the extraction of juice from fresh radishes. In shake flask experimetns, C. utilis ATCC 42416 grew well in CRJ and completed growth in 24 hrs at $30^{\circ}C$ and 200 rpm. Maximum cell dry weight obtainable from a liter of CRJ(1.0% sugar $DCRJ{\times}5$) was 21.5g, when the yeast was grown on CRJ diluted 5 times or more with tap water to make sugar content to be eual to or less than 1.0%. Supplementation of 5-fold diluted CRJ with some nutrients did not greatly influence the growth rate, yeast biomass production, or cell protein content significantly, indicating that CRJ itself was a good substrate for the production of biomass by C. utilis ATCC 42416.

단세포 단백질 자원으로 이용 가능한 균주로 알려진 Candida utilis ATCC 42416 균체를 생산하기 위하여 무를 파쇄하여 얻은 무즙을 배지로 균체배양 실험을 하였다. 무즙의 수용성 고형물과 총 당량은 각각 5.0~8.8 Brix와 3.5~6.5%였다. C. utilis ATCC 42416 균주를 무즙에 접종하여 $30^{\circ}C$에서 200rpm으로 진탕 배양하였을 때 24시간 이내에 증식이 끝났으며, 단위 소모당에 대한 최대 균체 생산량은 당농도 1% 회석 무즙에서 L당($5\;\times\;DCRI$) 21.5g의 건조균체를 생산하였다. 5배 회석무즙에 약간의 영양소 glucose(2.0%), yeast extract(0.2%), peptone(0.2%), ammonium sulfate(0.2%) 및 $KH_2PO_4(0.2%)$ 등을 각각 보강하여도 증식 속도, 균체생산 및 세포 단백질 함량 등에 차이가 거의 없어서 무즙자체가 C. utilis ATCC 42416 균체 생산에 좋은 기질이었음을 알 수 있다.

Keywords

References

  1. Biotechnology(2nd ed.) Smith,J.E.
  2. Korean. J. Appl. Microbiol. Bioeng v.1 Studies on the utilization of agricultural wastes. Acid hydrolysis of straws and the utilization of the hydrolysate Bae,M.;Kim,B.H.;Yoon,A.S.
  3. Koean J. Appl. Microbiol. Bioeng. v.4 studies on the fermentative utilization of cellulosic wastes. Production of yeast from the hydrolysate of rice straw, rice hull and corn starch pulp. Sung,N.K.;Shim,K.H.;Lee,C.S
  4. Korean J. Appl. Microbiol. Bioeng. v.7 Increasing the cell density in SCP production from cellulosic substrates Han,Y.W.
  5. Korean J. Appl. Microbiol. Bioeng. v.2 Studies on the utilization alcohol distiller's waste. Production of Saccharomyces cerevisiae cells from alcohol distilled waste of wheat flour. Yu,J.H.;Oh,D.H.;Yang,R.
  6. Korean J. Appl . Microbiol. Bioeng. v.10 Growth of the yeasts in alcohol distiller's waste of dried sweet potato for single cell protein production and BOD reduction. Lee,H.C.;Koo,Y.J.;Min,B.Y.;Lee,H.K.
  7. Korean. J. Appl. Microbiol. Bioeng. v.17 SCP production from mandarin orange peel press liquor Kang,S.K.;Sung,N.K.
  8. Korean. J. Appl. Microbiol. Bioeng. v.12 A study on the production of single cell protein from ginseng cake extract using Saccharomyces cerevisiae. Ju,H.K.;Cho,K.S.
  9. Korean. Appl. Microbiol. Bioeng. v.2 Studies on the production of yeast. Yeast production from the hydrolysate of sweet potato cake as a carbon source. Yang,H.C.;Choi,Y.J.;Sung,H.C.;
  10. Korean. J. Food Sci. Technol. v.23 Single cell protein production from Chinese cabbage juice. Lee,N.S.;Kyung,K.H.
  11. Korean. J. Food Sci. Technol. v.21 Changes of free sugars in kimch during fermentation Ha,J.H.;Hawer,W.D.;Kim,Y.J.;Nam,Y.J.
  12. Food composition Table.(3rd ed.) Rural Nutrition Institute
  13. Science. v.107 Calorimetric.determination of total carbohydrates Morris,D.L.
  14. Official methods of analysis. (14th ed.) A.O.A.C.
  15. Korean J Food Sci. Technol. v.21 Selection scheme of radish varieties to improve storage stabilites of femented pickled radish cubes with special reference to sugar content. Kim,K.J.;Kyung,K.H.;Myung,W.K.;Shim,S.T.;Kim,H.K.
  16. Appl. Microbiol. Biotechnol. v.20 Use of alfalfa residual juice as a substrate for propagation of the red yeast Phaffia rhodozyma Okagbue,R.N.;Lewis,M.J.
  17. Experientia v.29 The use of alfafa residual juice for production of single cell protein Paredes-Lopez,O;Camago,E.