Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

The Effect of Dietary n-3 and n-6 Polyunsaturated Fats on changes in Glucose, Non Esterified Fatty Acid and Fatty Acid Compositions in Serum of Rat Exposed to Stress.

N-3계 및 N-6계 지방산 식이가 스트레스에 노출된 흰 쥐의 혈당과 혈청 유리지방산 및 지방산 조성변화에 미치는 영향

  • 장문정 (국민대학교 사범대학 가정교육과)
  • Published : 1995.06.01
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Abstract

This study was designed to investigate the changes in energy substrates, glucose and non-esterified fatty acid(NEFA), and fatty acid compositions in serum, following physiolgical stress in rats fed diets containing various fatty acids. Forty two Sprague-Dawley strain male rats, weighing 108$\pm$2.1g, were fed 3 different experimental diets for 4 weeks. The diets were composed of 105 fat(w/w) of either corn oil(CO;18:2 n6:57%), plant perilla oil(PO;18:3 n3:59%), or tuna fish oil(FO;20:5 n3:17%%, 22:6 n3:19%). After 4 weeks of feeding, each group wa subdiveided into (a) control, (b) 2 min swim in ice-cold water. Animals wer decapitated 20min after commencing the swim; trunk blood, brain, liver and epididymal fat pad were obtained. The levels of serum corticosterone, glucose, NEFA, triglyceride, fatty acid compositions, brain serotonin and 5-hydroxyindoleacetic acid were determined. Basal levels of corticosterone na NEFA of serum were significantly lower in fish oil fed animals than those of any other oil fed animals. Compared to either perilla oil-fed or corn oil-fed rats, cold swim stress in fish oil fed rats produced significantly smaller NEFA and larger corticosterone responses. However, there was no significant difference in basal levels of serum glucose. Stress increased serum glucose levels slightly, and the amount of increment was larger in fish oil rats than those of any other oil fed rats than those of any other oil fed rats, although all the values were normal level. Dietary fats and stress did not affect serotonin metabolism. In additions, the composition of fatty acids in serum was significantly affected by the dietary compostion of fatty acids and stress. Stress induced decreases in monounsaturated fatty acid and non-polyunsaturated fatty acid concentration in either perilla oil fed or fish group, but did not in corn oil fed group. Stress resulted in changes in fatty acid metabolism similar to that associated with essential fatty acid(EFA) dificiency, when feeding animals n-3 fatty acids in diet. In conclusion, feeding fish oil was more effective to decrease NEFA in serum than feeding perilla oil or corn oil and improved lipid metabolism, when the rats were maintained in normal or exposed to stressful environment. However, the fact that feeding diet containing n-3 fatty acids decreased EFA status under stress suggests that the requirement of n-6 PUFA should be increased in these groups.

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References

  1. 한국영양학회지 v.23 영양과 관련된 질환의 현황과 대책 허갑범
  2. Nutr Rev v.50 Long-term implications for health Kromhout,D.;Dietary Fats
  3. Metabolism v.43 Altered fatty acid metabolism in patients with angiographically documented coronary artery disease Siguel,E.N;Lerman,R.H.
  4. Br Med J v.285 Fatty acid composition of serum lipids predicts myocardial infarction Miettinen,T.A.;Naukkarinen,V.;Huttunen,J.K.
  5. Nutr Rev v.46 Role of membrane lipids in metabolic regulation Berdanier,C.D.
  6. Nutr Cancer v.4 Cancerostatic effect of vegetable diets Siguel,E.N.
  7. Rev Pure Appl Phamacol Sci v.4 The regulation of prostaglandin biosynthesis by the manipulation of essential fatty acid metabolism Horrobin,D.F.
  8. Dietary fats Aging and nutritional requirements of essential fatty acid Siguel,E.N.;Schaefer,E.J.;Berae,J.(ed.)
  9. Crit Care Med v.8 Nutritional requirements of adult surgical patients Elwyn,D.H.
  10. Proc R Soc Med v.67 Stress hormones and lipid metabolism Kissebah,A.H.
  11. J Nutr Ment Dis v.147 Effects of quantified stressful stimuli on blood lipids in man Gittleman,B.;Shatin,L.;Bierenbaum,M.L.;Fleisohman,A.I.;Hayton,T.
  12. Pysiol Behavior v.39 A comparison of acute stress paradigms : Hormonal reponses and hypothalamic serotonin Paris,J.M.;Lerens,S.A.;Van de Kar L.D.;Urban,J.H.;Richardson,M.K.D.;Bethea,C.L.
  13. Can J Physiol Pharmacol v.65 Stress and cold-induced adrenocorticol responses in repetitedly immobilized or cold acclimated rats Yahata,T.;Murazum,K.;Kuroshima,A.
  14. Life Sci v.7 The effect of immobilization stress on the activity of central monoaminergic neurons Corrodi,H.;Fuxe,K.;Hokfelt,T.
  15. 한국영양학회지 v.27 고 트립토판 식이를 섭취한 마우스에서 immobilization 스트레스로 인한 면역변조와 serotonin 대사의 변화에 대한 연구 서경원;김혜리
  16. Biochem J v.200 Effects of chronic modification of dietary fat and carbohydrate on the insulin, corticosterone and metabolic responses of rats fed acutely with glucose, fructose or ethanol Brindley,D.N.;Cooling,J.;Glenny,H.P;Burditt,S.L.;Mckechnie,I.S.
  17. Biochem J v.200 Effects of chronic modification of dietary fat and carbohydrates in rats. Lawson,N.;Jennings,R.J.;Pollard,A.D.;Sturton,R.G.;Ralph,S.J.;Marsden,C.A.;Fears,R.;Brindlley,D.N.
  18. 한국영양학회지 v.27 어유의 Eicosapentaenoic acid 의 식이섭취가 성숙쥐의 혈청 및 간의 지질조성에 미치는 영향 정영진;박정숙;박화진;장유영
  19. Brain Res v.550 Enhanced responses to stress induced by fat feeding in rats : Relationship between hypothalamic noradrenaline and blood glucose Pascoe,W.S.;Smythe,G.A.;Storlien,L.H.
  20. Br J Pharmacol Chemother v.50 Rapid method for the detemination of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in small regions of rat brain Curzon,G.;Green,A.
  21. Poultry Sci v.63 A comparison of various procedures for determining total yolk lipid content Fletcher,D.L.;Britton,W.M.;Cason,J.A.
  22. J Lipid Res v.27 Direct transesterification of all classes of lipids in a one-step reaction Lepage,G.;Roy,C.C.
  23. Nutr Metabol v.16 The effect of different dietary levels of essential fatty acids on growth of the rat Alling,C.;Bruce,A.;Karlson,I.;Svennerholm,L.
  24. J Nutr v.114 The effect of essential fatty acid deficiency on basal respiration and function of liver mitochondria in rats Rafael,J.;Patzelt,J.;Schafer,H.;Elmadfa,I.
  25. Brain Res v.367 Brain serotonin and catecholamine responses to repeated stress in rats. De Souza E.B.;Van Loon G.R.
  26. Am J Clin Nutr no.SUP.57 The regulatory, informational, and immunomodulatory roles of fat fuels Newsholme,E.A.;Calder,P.;Yaqoob,P.
  27. Eur J Clin Nutr v.43 Comparison between habitual intake of polyunsaturated fatty acids and their concentrations in serum lipid fraction Houwelingen,A.C.V.;Kester,A.D.M.;Kromhout,D.;Hornstra,G.
  28. J lipid Res v.30 Fish oils and plasma lipid and lipoptotein metabolism in humans: A critical review Harris,W.S.
  29. 한국영양학회지 v.27 ω3계 및 ω6계 지방산 식이가 흰쥐가 모유와 혈청의 지방산 성분 및 비타민 E 수준에 미치는 영향 황혜진;정은정;이종호;지규만;이양자
  30. Diabetes care v.5 The role of behavior in diabetes care Surwit,R.S.;Scovern,A.W.;Feinglos,M.N.
  31. Diabetologia v.35 The effects of sympathetic nervous system activation and psychological stress on glucose matabolism and blood pressure in subjects with type 2 (non-insulin-dependent) diabetes mellitus Bruce,D.G.;Chisholm,D.J.;Storlien,L.H.;Kraegen,E.W.;Smythe,G.A.
  32. Life Sci v.18 Effects of L-tryptophan and restraint stress on hypothalamic and setotonin turnover and pituitary TSH and prolactin release in rats Muller,G.P.;Twohy,C.P.;Chen,H.T.;Advis,J.P.;Meites,J.
  33. Catecholamines and stress: Recent advaces Interaction of serotonin and catecholamines in individual brain nuclei in adrenocortical activity regulation during stress Culman,J.;Kvetnansky,R.;Kiss,A.;Mezey,E.;Murgas,K.;Usdin,E.(ed.);Kvetnansky,R.(ed.);Kopin,I.J.(ed.)
  34. Life Sci v.17 Effect of immobilization stress on serotonin content and turnover in regions of the rat brain Morgan,W.W.;Rudeen,P.K.;Pfeil,K.A.
  35. Am J Clin Nutr v.24 Effect of adrenal hormones and stress on brain serotonin Curzon,G.
  36. J Neurochem v.20 Effects of food deprivation and immobilization on tryptophan and other amino acids in rat brain Knott,P.J.;Joseph,M.H.;Curzon,G.
  37. Biol Chem v.259 The permissive effects of glucocorticoid on hepatic gluconeogenesis Chan,T.M.
  38. Clin Invest v.74 Role of counterregulatory hormones in the catabolic response to stress Gelfand,R.A.;Matthews,D.E.;Bier,D.M.
  39. Clin Sci v.64 The influence of different type of ω-3 polyunsaturated fatty acids on blood lipids and platelet function in healthy volunteers Sanders,T.;Roshai,F.
  40. Metabolism v.38 In vitro study of human skeletal muscle strips : Effect of non-esterified fatty acid supply on glucose storage Argyraki,M.;Wright,P.D.;Venables,C.W.
  41. Metabolism v.42 Peripheral and hepatic insulin sensitivity in non-insulin-dependent diabetes mellitus: Effect of nonesterified fatty acids Walker;Agius,L.;Orskiv,H.;Alberti,K.G.M.M.
  42. Physiol Behav v.26 Adrenal hormons and brain catecholamines response to morning and afternoon immobilization stress in rats Torrelas,S.;Guaza Borrel,J.;Borrel,S.