Journal of Microbiology and Biotechnology
- Volume 5 Issue 2
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- Pages.92-95
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- 1995
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Alginate Lyase Production of Halophilic Pseudomonas sp. by Recombinant Escherichia coli
- Kong, In-Soo (Department of Biotechnology and Bioengineering. National Fisheries University of Pusan) ;
- Kim, Young-Ok (Department of Biotechnology and Bioengineering. National Fisheries University of Pusan) ;
- Kim, Jin-Man (Department of Biotechnology, Yosu National Fisheries University) ;
- Kim, Sung-Koo (Department of Biotechnology and Bioengineering. National Fisheries University of Pusan) ;
- Oh, Doo-Hwan (Bioproducts Research Center, Yonsei University) ;
- Yu, Ju-Hyun (Bioproducts Research Center, Yonsei University) ;
- Kong, Jal-Yul (Department of Biotechnology and Bioengineering. National Fisheries University of Pusan)
- Published : 1995.04.01
Abstract
Halophilic Pseudomonas sp.W7 isolated from laver in the southem sea of Korea showed alginate lyase activity. Gene (aly) encoding alginate lyase was cloned in E.coli JM83 and the N-terminal amino acid sequence of the enzyme was determined after purificaion. The recombinant enzyme has been shown to have a molecular weight of about 40kDa after 12% SDS-polyacrylamide gel electrophoresis.