Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhanced Essential Oil Formation by Two-phase Culture of Mentha piperita Cells in Shake Flask and Air-lift Bioreactors

  • Kim, Teresa (Department and Institute of Genetic Engineering, Kyung Hee University) ;
  • Kim, Tae-Yong (Department and Institute of Genetic Engineering, Kyung Hee University) ;
  • Bae, Geun-Won (Department and Institute of Genetic Engineering, Kyung Hee University) ;
  • Chae, Young-Am (Research Center for New Bio-Materials in Agriculture) ;
  • Lee, Hyong-Joo (Research Center for New Bio-Materials in Agriculture) ;
  • Chung, In-Sik (Department and Institute of Genetic Engineering, Kyung Hee University)
  • Published : 1995.12.01
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Abstract

Effect of two-phase culture on Mentha piperita cell growth and essential oil formation was investigated using shake flask and air-lift bioreactors. LiChroprep RP-B(RP-B) addition did not impair M. piperita cell growth, but resulted in stimulated formation of essential oils and increased ratios of extracellular oil to intracellular oil formation. However, the combined use of RP-B and chitosan elicitor was not synergistic. Volumetric productivity of essential oils in RP-B treated culture using cell-recycled air-lift bioreactor was $6.9\;\mu\textrm{g}/l{\cdot}day$ which was substantially higher than that obtainable from the control. Our results demonstrate the potential of a second phase to enhance overall productivity for M. piperita cell culture.

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