BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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N-methyl-N'-nitro-N-nitrosoguanidine Reduces the Intracellular Calcium Level Through NAD Depletion in NIH3T3 Cells

  • Yoon, Yoo-Sik (Department of Biological Science, Korea Advanced Institute of Science and Technology) ;
  • Shin, In-Cheol (Department of Biological Science, Korea Advanced Institute of Science and Technology) ;
  • Kim, Jin-Woo (Department of Biological Science, Korea Advanced Institute of Science and Technology) ;
  • Kang, Ke-Won (Department of Biological Science, Korea Advanced Institute of Science and Technology) ;
  • Joe, Cheol-O (Department of Biological Science, Korea Advanced Institute of Science and Technology)
  • Received : 1995.04.03
  • Published : 1995.09.30
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Abstract

The effect of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) on the intracellular $Ca^{2+}$ level was studied in NIH3T3 fibroblast cells. A reduction of the intracellular $Ca^{2+}$ level was observed after exposure to 300 ${\mu}m$ MNNG. However, the intracellular level of $IP_3$, a well-known regulator of $Ca^{2+}$ release from internal storage, was not changed by MNNG treatment. Instead, a reduction of the intracellular NAD level was observed. NAD as well as $IP_3$ stimulated intracellular $Ca^{2+}$ release from permeabilized cells. The treatment of 3-aminobenzamide, which inhibited the MNNG-induced reduction of the NAD level, also prevented the MNNG-induced decrease of the $Ca^{2+}$ level. Our data suggest that MNNG reduces the intracellular $Ca^{2+}$ level by NAD depletion in NIH3T3 cells.

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