BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Identification of Isoleucine-Accepting tRNA in Maize Mitochondria

  • Park, Young-In (Department of Genetic Engineering, Korea University) ;
  • Lee, Byung-Chul (Department of Genetic Engineering, Korea University) ;
  • Chang, Hyo-Ihl (Department of Genetic Engineering, Korea University) ;
  • Moon, A-Ree (College of Pharmacy, Duksung Women's University)
  • Received : 1995.05.03
  • Published : 1995.11.30
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Abstract

Maize mitochondrial tRNAs for isoleucine have been isolated using a putative $tRNA^{Ile}$ gene probe which has been previously isolated and characterized. It contains the 5'-CAT anticodon which would normally recognize the AUG methionine codon. The nucleotide sequence of one of these tRNAs has been partially determined, and contains a modified nucleotide at the first position of the anticodon. This type of posttranscriptional modification event could change the specificity of amino acid acceptance of a tRNA, unlike that deduced from the corresponding gene. An aminoacylation experiment also demonstrated that these purified tRNAs have isoleucine acceptance activity but no methionine-accepting activity.

Keywords

References

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