BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Modulation of Phytotropin Receptors by Fluoride and ATP

  • Received : 1995.10.14
  • Published : 1995.11.30
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Abstract

Treatment of microsomal vesicles isolated from etiolated Pisum sativum L cv. Alaska epicotyl tissue with agents inhibiting protein dephosphorylation, namely NaF and/or ATP, resulted in increased binding of the phytotropin NPA to the putative auxin efflux carriers localized on the plasma membrane. The phytotropin effect was especially conspicuous if the vesicles were simultaneously treated with Triton X-100. Kinetic analysis of the binding indicated the existance of two distinct sites for NPA, each having different affinities. Increased binding of the phytotropin to the membrane where protein dephosphorylation was inhibited was attributable to the increased ligand affinity of both sites. Treatment of tissue segments with flubride was found to enhance in vivo auxin transport. Implications of covalent modification of the auxin efflux carrier complex for the regulation of membrane transport of auxin molecules are discussed.

Keywords

References

  1. Plant Sci. Lett. v.31 Depta, H.;Eisele, K.H.;Hertel, R. https://doi.org/10.1016/0304-4211(83)90055-X
  2. Biochem. Biophys. Res. Comm. v.157 Fujita-Yamaguchi, Y.;Kathuria, S. https://doi.org/10.1016/S0006-291X(88)80967-7
  3. Z. Pflanzenphysiol. v.112 Hertel, R. https://doi.org/10.1016/S0044-328X(83)80062-2
  4. Planta v.157 Hertel, R.;Lomax, T.L.;Briggs, W.R. https://doi.org/10.1007/BF00405182
  5. Planta v.142 Jacobs, M.;Hertel, R. https://doi.org/10.1007/BF00385113
  6. Plant Hormone receptors Kang, B.G.;Klambt, D.(ed.)
  7. Progress in Plant Growth Regulation Kang, B.G.;Park, W.J.;Nam, M.H.;Hertel, R.;Karssen, C.M.(ed.);Van Loon, L.C.(ed.);Vreugdenhil, D.(ed.)
  8. Phytochem. v.15 Katekar, G.F. https://doi.org/10.1016/S0031-9422(00)88906-2
  9. Aust. J. Plant Physiol. v.4 Katekar, G.F.;Geissler, A.E. https://doi.org/10.1071/PP9770321
  10. Plant Physiol. v.60 Katekar, G.F.;Geissler, A.E. https://doi.org/10.1104/pp.60.6.826
  11. Plant Physiol. v.66 Katekar, G.F.;Geissler, A.E. https://doi.org/10.1104/pp.66.6.1190
  12. Biochem. J. v.273 MacKintosh, C.;Coggins, J.;Cohen, P. https://doi.org/10.1042/bj2730733
  13. Planta v.187 Michalke, W.;Katekar, G.F.;Geissler, A.E.
  14. Biochem. Biophys. Res. Comm. v.171 Pesi, R.;Villa-Moruzzi, E. https://doi.org/10.1016/0006-291X(90)91402-E
  15. Planta v.154 Refeno, G.;Ranjeva, R.;Boudet, A.M. https://doi.org/10.1007/BF00387863
  16. Plant Hormones and their Role in Plant Growth and Development Rubery, P.H.;Davies, P.J.(ed.)
  17. Planta v.150 Sussman, M.R.;Goldsmith, M.H.M.
  18. Biochem. Biophys. Res. Comm. v.135 Tamaoki, T.;Nomoto, H.;Takahashi, Y.;Kato, Y.;Motimoto, M.;Tomita, F. https://doi.org/10.1016/0006-291X(86)90008-2
  19. J. Plant Physiol. v.140 Yoon, I.S.;Kang, B.G. https://doi.org/10.1016/S0176-1617(11)80822-9
  20. Proc. Natl. Acad. Sci. USA v.89 Zettl, R.;Feldwisch, J.;Boland, W.;Schell, J.;Palme, K. https://doi.org/10.1073/pnas.89.2.480