한국균학회지 (The Korean Journal of Mycology)

한국균학회 (The Korean Society of Mycology)
권호 표지

Pseudomonas cepacia 균주가 생산하는 항진균성 Cyclic Lipopeptide의 생물학적 및 물리 화학적 특성

Biological and Physico-chemical Properties of Antifungal Cyclic Lipopeptides Produced by Pseudomonas cepacia Strains

  • 김성호 (동국대학교 응용생물학과) ;
  • 이민웅 (동국대학교 응용생물학과)
  • 발행 : 1996.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

토양으로부터 항진균 활성을 나타내는 5종류의 Pseudomonas cepacia 균주 AF027, AF069, AF2001, AF2011, SD02가 분리되었으며 이들은 각각 cyclic lipopeptide계 항진균 물질 CLP027A, CLP069A, Cepacidine A, CLP2011A, CLP02A를 생산하였다. 조사된 P cepacia 균주들은 배지중에 함유된 질소원 및 탄소원의 종류에 따라 항진균 물질 생산성에 현저한 차이가 있었는데, 질소원으로서는 polypeptone-S, 탄소원으로서는 glucose와 fructose가 생산성 증대에 가장 효과적이었다. 항진균물질 $CLP069A_1$과 $CLP069A_2$는 각각 분자량이 1215와 1199이며, 아미노산 조성, UV spectrum, IR spectrum 및 항진균 스펙트럼에 있어서, Xylocandin $A_1,\;A_2$와 일치하였으며, CLP027 $A_1$과 $A_2$ 또한 이러한 물리 화학적 특성이 Cepacidine A와 완전히 일치함으로써 동일 물질로 생각된다. $CLP2011A_1,\;A_2$와 $CLP02A_1,\;A_2$는 아미노산 조성 및 항진균 스펙트럼이 Xylocandin $A_1,\;A_2$와 다르고, Cepacidine $A_1,\;A_2$와는 항진균 스펙트럼에서 현저하게 차이가 났다. CLP2011A, CLP02A가 분자량, 아미노산 조성, UV spectrum, IR spectrum에서 Cepacidine A와 동일하지만 항진균 스펙트럼이 상이한 것은 이들이 다른 물질임을 시사하며, 이는 aliphatic side chain이 Cepacidine A의 그것과 다른 데에서 비롯된 분자구조의 상이함에서 기인된 것으로 추론된다.

Five strains AF027, AF069, AF2001, AF2011 and SD02 of Pseudomonas cepacia were isolated from soil, and the antifungal cyclic lipopeptides(CLP) i.e, CLP027A, CLP069A, Cepacidine A, CLP2011A and CLP02A were produced from each strains, respectively. Nitrogen and carbon sources in media were proved to be important factors for the production of CLP and among them, polypeptone-S, glucose and fructose were the most effective. It appeared that compounds CLP027A and CLP069A were identical with Cepacidine A and Xylocandine A, respectively. contain aspartic acid as amino acid component, are differentiated from Xylocandine A containing asparagine. Although molecular weight, amino acid composition and UV spectrum of CLP2011A and CLP02A are same with those of Cepacidine A, it is postulated that these compounds are not identical with Cepacidine A when the antifungal spectra and antifungal activity were compared to those of Cepacidine A.

키워드

참고문헌

  1. Kor. J. Appl. Microbiol. Biotechnol. v.23 Pseudomonas aeruginosa KGM-100이 생산하는 항생물질의 특성 및 구조 김경석;홍수형;이은주;박용북;박용태;하지홍
  2. Kor. J. Appl. Microbiol. Biotechnol. v.21 신규 항진균 물질 AF-011A의 동정, 정제 및 물리 화학적 특성 김성호;현봉철;서정우;김창완;연창석;이덕근;김광표;정재경;임융호;이철훈
  3. Kor. J. Appl. Microbiol. Biotechnol. v.21 신규 항진균 물질 AF-011A의 생물학적 활성 및 구조분석 서정우;임융호;김성호;현봉철;김창완;연창석;이덕근;김광표;정재경;이철훈
  4. Kor. J. Appl. Microbiol. Biotechnol. v.19 Bacillus subtilis subsp. krictiensis로 부터 항진균물질 KRF-011의 생산을 위한 발효조건 및 돌연변이 연구 손광희;권혜경;이항우;복성해
  5. Kor. J. Appl. Microbiol. Biotechnol. v.23 식물 뿌리썩음병을 유발하는 Fusarium solani에 대한 Ps-edudomonas 속의 생물학적 방제기작 이은주;김경석;홍수형;하지홍
  6. Kor. J. Appl. Microbial. Biotechnol. v.23 항진균성 길항세균 Bacillus subtilis YBL-7의 종자피막용 포자체의 생산과 발아조건 장종원;김상달
  7. Kor. J. Appl. Microbial. Biotechnol. v.23 길항세균 Bacillus subtilis YBL-7의 건조포자체의 종자피막화에 의한 생물학적 방제 장종원;김상달
  8. J. Appl. Bacterial. v.61 Occurrence of fungal-inhibiting Pseudomonas on caryopses of Tripsacum dactyloides L. and its implications for seed survival and agricultural application Anderson, R.C.;Liberta, A.E.
  9. The Journal of Antibiotics v.41 Bacillomycins Fb and Fc: Isolation and characterization Besson, F.;Michel, G.
  10. The Journal of Antibiotics v.40 Xylocandin: a new complex of antifungal Peptides. II. Structural studies and chemical modifications Bisacchi, G.S.;Hockstein, D.R.;Koster, W.H.;Parker, W.L.;Rathnum, M.L.;Unger, S.E.
  11. Antimicrob. Agents Chemother. v.29 Characterization of an antibiotic produced by a strain of Pseudomonas fluorescens inhibitory to Gaeumannomyces graminis var. tritici and Pythium spp Gurusiddaian, S.;Weller, D.M.;Sarkar, A.;Cook, R.J.
  12. Pyoverdin production by the plant growth beneficial Pseudomonas strain 7NSK$_2$: Economic significance in soil;Iron nutrition and interactions in plants Hofte, M.;Seong, K.Y.;Jurkevitch, E.;Verstraete, W.;Chen, Y.(ed.)Hadar, Y.(ed.)
  13. Ann. Phytopath. Soc. Japan v.55 Role of antibiotic production in suppression of radish damping-off by seed bacterization Homma, H.;Suzui, T.
  14. Soil Bioi. Biochem. v.21 Production of antibiotics by Pseudomonas cepacia as an agent for biological control of soil borne plant pathogens Homma, Y.;Sato, Z.;Hirayama, F.;Konno, K.;Shiarhama, H.;Suzui, T.
  15. Tetrahedron Letters v.23 Structures of $\beta$-amino acids in antibiotics Iturin A Isokai, A.;Takayama, S.;Murakoshi, S.;Suzuki, A.
  16. Amer. Phytopathol. Soc. v.78 Biological control of blue mold and gray mold on apple and pear with Pseudomonas cepacia Janisiewicz, W.J.;Roitman, J.
  17. Appl. Environ. Microbiol. v.56 Isolation and characterization of a Pseudomonas strain that restricts growth of various phytopathogenic fungi Jayaswal, R.K.;Fernandez, M.A.;Schroeder, R.G. III
  18. Ann. Rev. Phytopathol. v.24 Siderophores: Their biochemistry and possible role in the biocontrol of plant pathogens John Leong
  19. Kor. J. of Mycol. v.22 Establishment of rhizosphere microbes for plant protection on soil-borne diseases Kim, S.I.;Lee, M.W.
  20. Cur. Microbiol. v.4 Pseudomonas siderophores: A Mechanism explaning disease-suppressive soils Kloepper, J.W.;Leong, J.;Teintze, M.;Schroth, M.N.
  21. The Journal of Antibiotics v.47 Cepacidine A, a novel antifungal antibiotic produced by Pseudomonas cepacia. I. Taxonomy, production, isolation and biological activity Lee, C.H.;Kim, S.H.;Hyun, B.C.;Suh, J.W.;Yon, C.S.;Kim, C.O.;Lim, Y.H.;Kim, C.S.
  22. Microbiol. Rev. v.43 Secondary metabolites of the fluorescent Pseudomonas Leisinger, T.;Margraff, R.
  23. The Journal of Antibiotics v.47 Cepacidine A, a novel antifungal antibiotic produced by Pseudomonas cepacia. II. Physico-chemical properties and structure elucidation Lim, Y.H.;Suh, J.W.;Kim, S.H.;Hyun, B.C.;Kim, C.S.;Lee, C.H.
  24. Biochemical tests for identification of medical bacteria McFaddin, J.F.
  25. Antifungal drugs;Antibiotics in laboratory medicine McGinnis, M.R.;Rinaldi, M.G.;Lorian, V.(ed.)
  26. Appl. Environ. Microbiol. v.58 Pseudomonas cepacia suppression of sunflower wilt fungus and role of antifungal compounds in controlling the disease McLoughlin, T.J.;Quinn, J.P.;Bettermann, A.;Bookland, R.
  27. The Journal of Antibiotics v.40 Xylocandin: A new complex of antifungal peptides. I. Taxonomy, isolation and biological activity Meyers, E.;Bisacchi, G.S.;Dean, L.;Liu, W.C.;Minassian, B.;Slusarchyk, D.S.;Sykes, R.B.;Tanaka, S.K.;Trejo, W.
  28. The Journal of Antibiotics v.40 Mulundocandin, a new lipopeptide antibiotic. II. Structure elucidation Mukhopadhyay, T.;Ganguli, B.N.
  29. Pseudomonadaceae;Bergey's manual of systematic bacteriology. Vol. 1 Palleroni, N.J.;Krieg, N.R.(ed.);Holt, J.G.(ed.)
  30. Amer. Phytopathol. Soc. v.80 Population Dynamics of Pseudomonas cepacia in the Pea Spermosphere in Relation to Biocontrol Pythium Parke, J.L.
  31. Soil Biol. Biochem. v.22 Relation between soil mass adhering to pea taproots and recovery of Pseudomonas fluorescens from the rhizosphere Parke, J.L.;Liddell, C.M.;Clayton, M.K.
  32. Soil Bioi. Biochem. v.19 Bioassay for studying the role of siderophores in potato growth stimulation by Pseudomonas spp. in short potato rotations Peter, A.;Bakker, H.M.;Bakker, A.W.;Marugg, J.D.;Weisbeek, P.J.;Schippers, B.
  33. Eur. J. Biochem. v.118 Structure of Bacillomycin D, a new antibiotic of Iturin group Peypoux, F.;Besson;Michel, E.G.;Delcambe, L.
  34. The Journal of Antibiotics v.37 Structure of Bacillomycin D and Bacillomycin L peptidolipid antibiotics from Bacillus subtilis Peypoux, F.;Pommier, M.T.;Das, B.C.;Besson, Delcambe, F.L.;Michel, G.
  35. Appl. Microbiol. Biotechnol. v.34 Production and composition of phenylpyrrole metabolites produced by Pseudomonas cepacia Roitman, J.N.;Mahoney, N.E.;Janisiwicz, W.J.
  36. J. Agric. Food Chem. v.38 A new chlorinated phenylpyrrole antibiotic produced by the antifungal bacterium Pseudomonas cepacia Roitman, J.N.;Mahoney, N.E.;Janisiwicz, W.J.;Benson, M.
  37. Appl. Environ. Microbiol. v.53 Evaluation of Pseudomonas fluorescens for suppression of sheath rot disease and for enhancement of grain yields in rice(Oryza sativa L.) Sakthivel, N.;Gnanamanickam, S.S.
  38. Soil Biol. Biochem. v.23 Growth, survival, and root colonization of plant growth beneficial Pseudomonas fluorescens ANP15 and Pseudomonas aeruginosa 7NSK2 at different temperatures Seong, K.Y.;Hofte, M.;Boelens, J.;Verstraete, W.
  39. The Journal of Antibiotics v.45 Isolation and characterization of new peptide antibiotics, Plusbacins A$_1$-A$_4$ and B$_1$-B$_4$ Shoji, J.;Hinoo, H.;Katayama, T.;Matsumoto, K.;Tanimoto, T.;Hattori, T.;Higashiyama, I.;Miwa, H.;Motokawa, K.;Yoshida, T.
  40. The Journal of Antibiotics v.45 Structures of new peptide antibiotics, A$_1$-A$_4$ and B$_1$-B$_4$ Shoji, J.;Hinoo, H.;Katayama, T.;Nakagawa, Y.;Ikenishi, Y.;Iwatani, K.;Yoshida, T.
  41. Appl. Environ. Microbiol. v.56 Production of the antibiotic phenazine-1-carboxylic acid by fluorescent Pseudomonas species in the rhizosphere of wheat Thomashow, L.S.;Weller, D.M.;Bomsall, R.F.;Pierson, L.S. III
  42. The EMBO Journal v.8 Cyanide production by Pseudomonas fluorescens helps suppress black root of tobacco under gnotobiotic conditions Voisard, C.;Keel, C.;Haas, D.;Defago, G.
  43. The Journal of Antibiotics v.36 Iturin A$_L$-a long chain Iturin A possessing an unusual high content of C10-$\beta$-amino acids Winkelmann, G.;Allgaier, H.;Lupp, R.;Jung, G.