Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation of 3,4-Dihydroxycinnamic Acid with Antimicrobial Activity from Bark of Aralia elata

두릅수피에서 항미생물 활성을 갖는 3,4-Dihydroxycinnamic Acid의 분리

  • Ma, Seung-Jin (Department of Food Science and Technology, Chonnam National University) ;
  • Kuk, Ju-Hee (Department of Food Science and Technology, Chonnam National University) ;
  • Ko, Byoung-Seob (Korea Institute of Oriental Medicine) ;
  • Park, Keun-Hyung (Department of Food Science and Technology, Chonnam National University)
  • Published : 1996.06.30
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Abstract

The methanol extract of Aralia elata bark showed antimicrobial activities against bacteria, yeast and fungi. The active components were successively purified with solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, silica gel partition column chromatography and HPLC. The active substances were separated with HPLC where 1% acetic acid-MeOH (60 : 40, v/v) was used as mobile phase. The isolated active substance ($t_r$ 17.1 min) was identified as trans-3,4-dihydroxycinnamic acid by $MS,\;^1H-NMR\;and\;^13C-NMR$.

두릅나무(Aralia elata $S_{EEMANN}$) 껍질의 MeOH추출물이 세균과 호모, 곰팡이 등에 대한 항미생물 활성을 보여 solvent fractionation, Silica gel adsorption chromatography, Sephadex LH-20 column chromatography, silica gel partition chromatography, HPLC 등의 기법으로 활성물질을 순차 정제하여 얻어진 활성물질을 1% acetic acid-MeOH (60 : 40, v/v)용매계의 HPLC에 의해 분리하였다. 분리된 성분($t_r$ 17.1 min)의 활성본체를 구명하기 위하여 $MS,\;^1H-NMR\;and\;^13C-NMR$. 등을 이용하여 기기분석한 결과, trans-3,4-dihydroxycinnamic acid로 동정되었다. 3,4-dihydroxycinnamic acid가 두릅에서 항미생물 활성물질로서 분리, 동정된 것은 처음으로 생각된다.

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