BMB Reports
- Volume 29 Issue 2
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- Pages.122-126
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- 1996
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- 1976-670X(eISSN)
Sequence Verification of Synthetic Oligonucleotides by Exonuclease Digestion and Matrix Assisted Laser Desorption Ionization Mass Spectrometry
- Kim, Jin-Sung (Korea Basic Science Institute) ;
- Jang, Jung-Suk (Korea Research Institute of Chemical Technology) ;
- Choi, Jong-Soon (Korea Basic Science Institute) ;
- Chang, Yoon-Seok (Department of Chemistry, Hanyang University)
- Published : 1996.03.31
Abstract
A series of oligonucleotides were synthesized by automatic DNA synthesizer. The purity of crude products was checked and their molecular weights determined by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) with an accuracy of better than 0.05% deviation even without using an internal standard. This mass determining technology in combination with partial digestion of oligonucleotides by 5'- and 3'-exonuclease provides a straightforward and simple method to obtain sequence information of oligonucleotides. The extension of this technology to the sequencing of modified oligonucleotides and genomic DNA and RNA might become possible.
Keywords
- digestion;
- exonuclease;
- matrix assisted laser desorption ionization mass spectrometry;
- oligonucleotides;
- sequencing