BMB Reports
- Volume 29 Issue 2
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- Pages.133-136
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- 1996
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- 1976-670X(eISSN)
Detection of Cleavage Sites on 5S rRNA by Methidiumpropyl-EDTA-Iron(II)
- Kim, Sang-Bumn (Department of Chemistry, Seoul National University) ;
- Cho, Bong-Rae (Department of Chemistry, Seoul National University) ;
-
Lee, Young-Hoon
(Department of Chemistry, Korea Advanced Institute of Science and Technology)
;
- Park, In-Won (Department of Chemistry, Seoul National University)
- Published : 1996.03.31
Abstract
The affinity cleavage reagent Methidiumpropyl-EDTA-Iron(II) is applied to the structural analysis of 5S rRNA. Analysis of cleavage sites induced by MPE-Fe(II) on 5S rRNA shows that MPE intercalates easily between the unstable base pairs or into the bulges, thereby it strongly cuts the nucleosides nearby. The stable helical stems A, B, D and E as well as loop d are weakly cut. Most of the single-stranded loops are not cleaved. Based on the cleavage pattern of the 5S rRNA by MPE-Fe(II) and RNase V1, we suggest that MPE-Fe(II) may be used as a potential chemical probe in searching for the unstable helical regions of RNA, and for the sequences that appear to be involved in folding and distorting 5S rRNA.