Parasites, Hosts and Diseases
- Volume 34 Issue 3
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- Pages.211-213
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- 1996
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Electrophoretic variations of enzyme, GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4) in characterizing clones and isolates of Malaysian Plasmodium falciparum
- Ang, Hooi-Hoon (School of Pharmaceutical Sciences, University of Science Malaysia) ;
- Chan, Kit-Lam (School of Pharmaceutical Sciences, University of Science Malaysia) ;
- Mak, Joon-Wah (Malaria Division, Institute for Medical Research)
- Published : 1996.09.01
Abstract
Malaysian, Ajricn and Thai PZQsmodiumJnkipamm isolates were cultured in uiko by the Tracer and Jensen method (1976, 1977) and were later cloned by the limiting dilution method (Rosario, 1981), Forty-eight clones were obtained and were characterized by electrophoretic variations of GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4). It was found that they were pure clones because they possessed either GDH-1 or GDH-2 unlike their parent isolates which exhibited both GDH-1 and GDH-2.
Keywords
- Plasmodium falciparum;
- isolates;
- clones;
- NADP-dependent glutamate dehydrogenase;
- electrophoretic variations