Photoreactivity of Anthraquinones for the Analysis of Ginsenosides Using Photoreduction Fluorescence Detection-HPLC

  • Published : 1996.12.01

Abstract

The photoreactivity of twelve anthraquinone derivatives was examined to evaluate its usefulness as a photo-reagent for the analysis of ginsenosides using photoreduction fluorescence (PRF) detection method. Among the tested compounds, 2-tert-butylandthraquinone (TBAQ), 2-chloroanthraquinone (CAQ) and anthraquinone (AQ) showed good characteristics as photoreagents. The detection limits of ginsenoside $Rg_{1}$PRF-HPLC method using TBAQ, CAQ or AQ as a photo-reagent were found to be ca. 35 ng, 50 ng and 50 ng, respectively.

Keywords

References

  1. Photochemistry and Photobiology v.17 Studies on some intermediates and products of the photoreduction of 9,10-anthraquinone Carlson,S.A.;Hercules,D.M.
  2. J. Phys. Chem. v.89 Porton transfer and hydrogen bonding in the internal conversion of $S_1$ anthraquinones Flom,S.R.;Barbara,P.F.
  3. Anal. Chem. v.54 Photoreduction-fluorescence detection of aliphatic alcohol, aldehydes and ethers in liquid chromatography Gandelman,M.S.;Birk,J.W.
  4. J. Chromatogr. v.282 Liquid chromatographic detection of cardiac glycosides and saccharides based on the photoreduction of anthraquinone-2,6-disulfonate Gandelman,M.S.;Birks,J.W.
  5. Anal. Chim. Acta. v.155 Liquid Chromatographic detection of cardiac glycosides and saccharides and hydrocortisone based on the Photoreduction of 2-tert-butylanthraquinone Gandelman,M.S.;Birks,J.W.
  6. Arch. Pharm. Res. v.15 no.4 Analysis of Ginseng saponins by HPLC with photoreduction fluorescence detection Kim,B.Y.;Lee,M.Y.;Cho,K.H.;Park,J.H.;Park,M.K.
  7. J. Phys. Chem. v.87 Photochemistry of 9,10-anthraquinone-2-sulfonate in solution 1. Intermediates and mechanism Loelt,I.;Treinin,A.;Linschitz,H.
  8. J. Chem. Soc., Faraday Trans. 2 v.82 Photochemistry of 9,10-anthraquinone-2,6-disulphonate Moore,J.N.;Philips,D.;Nakashima,N.;Yoshihara,K.
  9. J. Chem. Soc., Faraday Trans. 2 v.83 no.8 Photophysics and Photochemistry of sulphonated derivatives of 9,10-anthraquinone Moore,J.N.;Philips,D.;Nakashima,N.;Yoshihara,K.
  10. J. Liq. Chromatogr. v.18 no.10 High performance liquid chromatographic determination of ginsenosides using photoreduction fluorescence detection Park,M.K.;Kim,B.K.;Park,J.H.;Shin,Y.G.;Cho,K.H.
  11. Yakhakhoeji v.40 Analysis of Saikosaponins by HPLC with photoreduction flourescence detection Shin,Y.G.;Cho,K.H.;Kwon,S.J.;Do,Y.M.;Hwang,G.S.;Park,J.H.;Park,M.K.