Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Restriction pattern of the nucleic acid of Synechococcus sp. cyanophage

  • Park, Jong-Geun (Department of Biology, Hanyang University) ;
  • Kim, Min (Department of Biology, Hanyang University) ;
  • Choi, Yong-Keel (Department of Biology, Hanyang University/Research Center for Molecular Microbiology, Seoul National University) ;
  • Yoon, Sung-Nyo (Department of Biology, Hanyang University)
  • Published : 1996.03.01
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Abstract

The nucleic acid of Synechococcus sp. cyanophage was identified as double-stranded DNA by the result of digestion with enzymes such as exonucleases, DNase, and S1 nuclease, and by acridine orange staining. The cyanophage DNA was cleaved with several restriciton ehdonucleases such as ApaI, BamHI, Bg/II, HaeIII, Eco RI, HindIII, PstI, AND aPAI gave the clearest sets of bands on agarose gels and the fragment numbers for each were 12, 20, 29, 20, and 7, respectively. The sums of the size from Bam HI and PstI digestions were estimated approximately 227$\pm$4 kb, which are in agreement with the result of the pulsed field gel electrphoresis. This virus is thought to have the largest genosome among those of known cyanophages, which corresponds to the largest haed ot 90 nm when compared with the head sizes of cyanophages discovered since 1963.

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References

  1. Virology v.47 Comparison of the structures of blue-green algal virus LPP-1M and LPP-2 and bacteriophage T7 Adolph, K.W.;R. Haselkorn
  2. J. Phycol. v.4 Simple conditions for growth of unicellular blue-green algae on plates Allen, M.M.
  3. Pulsed field gel electrophoresis: a practical guide Birren, B.;E. Lai
  4. Bacteriol. Rev. v.31 Ultrastructure of bacteriophage and bacteriocine Bradley, D.E.
  5. J. Bacteriol. v.139 Characteristics of Anabaena variabilis influencing plaque formation by cyanophage N-1 Currier, T.C.;C.P. Wolk
  6. J. Microbiol. v.32 Isolation and partical characterization of a new Escherichia coli bacteriophage E3 Hong, S.H.;N.Y. Cho;G. Jeong
  7. Kor. J. Lim. v.25 Isolation of blue-green algae dominant at 5 reservoirs in Kyonggi-Do Kim, M.;Y.K. Choi
  8. J. Microbiol. v.30 Isolation and identification of cyanophage from eutrophic water Kim, M.;Y.K. Choi
  9. J. Microbiol. v.32 Influence of the photosynthesis of Synechococcus sp. on the development of its cyanophage Kim, M.;Y.K. Choi
  10. Virology v.204 A new Synechococcus cyanophage from a reservoir in Korea Kim, M.;Y.G. Choi
  11. Virology v.49 Physical properties of blue-green algal virus SM-1 and its DNA Mackenzie, J.M.;R, Haselkorn
  12. Encyclopedia of Virology Cyanobacteria bacteriophages Martin, E.U.;R.G. Webster(ed.);A. Granoff(ed.)
  13. Introduction to Environmental Microbiology Mitchell, R.
  14. Bacteriol. Rev. v.37 Cyanophages-viruses attacking blue-green algae Padan, E.;M. Shilo
  15. A Practical Guide to Molecular Cloning Perbal, B.
  16. Light Microscopy in Biology Fluorescence microscopy Ploem, J.S.;A.J. Lacey(ed.)
  17. Molecular cloning : a laboratory manual(2nd ed.) Sambrook, J.;E.F. Fritsch;T. Maniatis
  18. J. Virol. v.6 LPP-1 infection of the blue-green alga Plectonema boryanum, Ⅰ.Electron microscopy Sherman, L. A.;R. Haselkorn
  19. Nucleic Acids Res. v.14 Restriction endonuclease activity induced by NC-1A virus infection of chorella-like green alga Xia, Y.;D.E. Burbank;J.L. Etten
  20. Nucleic Acids Res. v.15 IL-3A virus infection of a chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity Xia, Y.;D.E. Burbank;L. Uher;J.L. Van Etten