Effect of Cosubstrate on tile Production of Poly(3-Hydroxybutyric-Co-3-Hydroxyvaleric) Acid from Glucose by Pseudomonas sp, HJ

Pseudomonas sp. HJ에 의한 포도당으로부터 Poly(3-Hydroxybutyric-Co-3-Hydroxyvaleric) Acid의 생합성에 대한 보조기질의 영향

  • 손홍주 (부산대학교 자연과학대학 미생물학과) ;
  • 고명선이상준 (부산대학교 자연과학대학 미생물학과 부산대학교 자연과학대학 미생물학과)
  • Published : 1996.10.01


Poly(3-hydroxybutyric-co-3-hydroxyvaleric) acid(PHB/HV) copolymer synthesis by Pseudomonas sp. HJ from glucose and cosubstrate was investigated. Taxonomic analysis suggested that Pseudomonas sp. HJ was best marched to Pseudomonas picketti having 78.8% similarity. Pseudomonas sp. HJ produced PHB/HV copolymer containing 60.8 mol% HV and 44.9 mol% HV when supplied with hexadecane and propionic acid as a cosubstrate, respectively. The HV composition in PHB/HV copolymer was controlled by varying the concentration of hexadecane and propionic acid. Propionic acid added after 24 hours of incubation was incorporated as the HV monomer in the PHB/HV copolymer up to 49.6 mol%.