Effect of Dietary Minerals and Ca-Regulating Hormones on Bone Enzyme Alkaline Phosphatase Activity

  • 발행 : 1996.06.01

초록

Parathyroid hormone(PTH) is known to stimulate bone resorption and to inhibit bone collagen synthesis. In contrast, as the evidence of stimulation of bone formation by PTH has recently been observed, the study on the role of PTH involved in osteoporosis draws remarkable attention. This study has dealt with the role of alkaline phoshatase(AP), a marker enzyme for bone formation and osteoblast action, Animals(BALS/cmice) were divided into three dietary groups(high and medium Ca and Ca-free) and hormones including PTH, calcitonin(CT), cholecalciferol(citamin D) were i.p. injected. AP in the serum and liver was measured using Sigma 221 alkaline buffer solutions containing 9mM of p-nitrophenyl phoshate. Enzume was reacted at 37$^{\circ}C$ for 10 minutes and the reaction was stopped by 1.8ml of 0.1N NaOH and measured at 410nm. We found that serum and liver AP activity was increased by low dietary Ac. Compared to the control, and serum Ap activity was enhanced by PTH and vitamin D regardless of the dietary Ca. On the other hand, liver AP activity was inhibited by OTH and vitamin D at all levels of dietary Ca. CT inhibited the action of PTH and vitamin D in the serum. But, the inhibition of PTH and vitamin D action by CT was not observed in the liver, unlike in the case of serum.

키워드

참고문헌

  1. Clin. Orthop. v.151 The cellular basis of bone resorption Chambers,T.J.
  2. J. Clin. Invest. v.44 Bone resorption in tissue culture. Factors influencing the response to parathyroid hormone Raisz,L.G.
  3. Endocrinology v.98 Hormonal control of collagen synthesis in vitro : effect of parathyroid hormone and calcification Dietrich,J.W.;Canalis,E.M.;Maina,D.M.;Raisz,L.G.
  4. Endocrinology v.110 Parathyroid hormone stimulates the bone apposition rate independently of its resorptive action : Differential effects of intermittent and continuous administration Tam,C.S.;Heersche,N.M.;Murray,T.M.;Parsons,J.A.
  5. Geriatrics v.8 Calcium and phosphorus intake in senile osteoporosis Paulsen,N.V.
  6. JADA v.88 The effect of diet and life style on bone mass in women Wardlaw,G.
  7. CMAJ v.136 Osteoporosis ; calcium and physical activity Alan,D.;Martin,C.
  8. Kor. J. Nutr. v.24 Factors influencing the bone status of Korean elderly women Kim,H.K.;Yoon,J.S.
  9. Kor. J. Nutr. v.27 The effect of Ca and vitamin D supplementation on bone metabolism in postmenopausal women Hong,H.O.;Yu,C.H.
  10. Am. J. Clin. Nutr. v.32 Bone status and fracture rates in two regions of Yugoslavia Matokovic,V.;Kostial,K.;Simonovic,L.;Buzin,R.;Brodarec,A.;Nordin,B.E.C.
  11. Nutrition and bone development Nutritional factors affecting mineral bomeostasis and mineralization in the term and preterm infant Hillman,L.S.;Simmons,D.J.(ed.)
  12. Endocrinology v.118 Osteoblastic cell mediate osteoclastic responsiveness to parathyroid hormone McSheehy,P.M.J.;Chembers,T.J.
  13. Proc. K. Ned. Akad. Wet.(Biol Med) v.C80 Effects of parathyroid hormone and a synthetic fragment(PTH 1-34) in bone in vitro Gaillard,P.J.;Wassenaar,A.M.;Wijhe Wheeler, M.Z.
  14. Kor. J. Endocrinology v.4 Parathyroid hormonal effect on bone formation and resorption in culture mouse calvarial tissues Chung,C.K.
  15. Hallym Univ. J.(Natural Sciences & Medicine) v.5 In vitro stimulation of bone formation and protein synthesis by parathyroid hormone in cultured neonatal mouse calvarial tissue Chung,C.K.
  16. Kor. J. Nutr. v.28 Effect of parathyroid hormone and calcitonin on the enzyme and mineral metabolism of bone cells and phosphorylation Chung,C.K.;Ha,K.S.
  17. Recent progress in hormone research Hormonal stimulation of bone formation Raisz,L.G.;Canalis,E.M.;Dietrich,J.M.;Kream,B.E.;Gwork,S.C.
  18. Anal. Biochm. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford,M.M.
  19. J. Clin. Ultrasound v.22 Values of serum alkaline phosphatase evaluating hyperplasia of parathyroid glands in chronic hemodialysis patients Huang,Y.Y.;Hsu,B.R.;Huang,B.Y.;Huang,H.S.;Huang,M.J.
  20. Acta Endocrinol. Copenh. v.127 Serum levels of intact parathyroid hormone and alkaline phosphatase correlate with cortical and trabecular bone loss in primary hyperparathyroidism Pfeilschifter,J.;Siegrist,E.;Wuster,C.;Blind,E.;Ziegler,R.
  21. J. Clin. Endcrinol. Metab. v.59 Clinical evaluation of bone turnover by serum osteocalcin measurements in a hospital setting Slovik,D.M.;Gundberg,C.M.;Neer,R.M.;Lian,J.B.
  22. Bone v.16 Calcium and vitamin D enriched diets increase and preserve vertebral mineral content in aging laboratory rats Schapira,D.;Linn,S.;Sarid,M.;Mokadi,S.;Kabala,A.;Sibermann,M.
  23. Intern. Med. v.32 Suppression of secondary hyperparathyroidism in chronic dialysis patients by single oral weekly dose of 1,25-dihydroxycholecalciferol Shigematsu,T.;Kawaguchi,Y.;Unemura,S.;Yamamoto,H.;Momose,M.;Yokoyama,K.;Wakabayashi,Y.;Ikeda,M.;Hasegawa,T.;Sakai,O.
  24. Clin. Orthop. v.293 Vitamin D status in Paget's bone disease. Effect of calcitonin therapy Nunziata,V.;Giannattasio,R.;di Giovanni, G.;Lettera,A.M.;Nunziats,C.A.
  25. Clin. Orthop. v.193 Effect of growth factors on bone cell replication and differentiation Canalis,E.
  26. Endocrine control of growth Growth hormone and the somatomedins Daughaday,W.H.;Daughaday,W.H.(ed.)
  27. Proc. Natl. Aca. Sci. USA v.71 Synthesis of nerve growth factor by L and 3T3 cells in culture Oger,J.;Arnason,B.G.W.;Pantozis,N.;Lehrich,J.;Young,M.
  28. J. Biol. Chem. v.255 Purification of a cartilage-derived growth factor Klagsbum,M.;Smith,S.
  29. Nature v.272 A human fibrosarcoma cell line producing multiplication stimulating activity (MSA) related peptides De Larco, J. E.;Todaro,G.J.
  30. Proc. Natl. Acad. Sci. USA v.75 Growth factors from murine sarcoma virus-transformed cells De Larco, J. E.;Todaro,G.J.
  31. JBC v.265 Cyclic AMP induces insulin-like growth factor I synthesis in osteoblast-enriched cultures McCarthy,T.L.;Centrella,M.;Canalis,E.
  32. Calcified Tissue Int. v.53 Combined treatment with calcitonin and 1,25(OH)₂D₃ for osteoporosis in women Staffan,A.V.;Lindgren,J.U.
  33. Nippon Naibupi Gakkai Zasshi v.67 The effect of estrogen and sex-steroids and thyroid hormone preparation on bone mineral density in senile osteoporosis-a comparative study of the effect of 1 alpha-hydroxycholecalciferol in senile osteoporosis Shiraki,M.;Orimo,H.