Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Enhanced Hydrolysis of Cellulose by Combination of Humicola insolens Cellulase with Immobilized Glucose Isomerase

  • Hur, Sung-Ho (Dept. of Food Technology, Dogeui Technical College) ;
  • Lee, Ho-Jae (Dept. of Food Technology, Dogeui Technical College) ;
  • Lee, Jae-Kwon (Dept. of Food Processing, Kyonggi University)
  • Published : 1996.06.01
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Abstract

To enhance cellulose hydrolysis f Humicola insolens cellulase (HIC) was empolyed with immobilized glucose isomerase(IGI). Optimun pH and temperature for HIC were 6.5 and 55$^{\circ}C$, respectively, whereas those for IGI were 7.0 and 60$^{\circ}C$, respectively. Optimun loading size of IGI was 200mg(130 untis) with 15units of HIC. Reaction conditions were determined to be as follows: 55$^{\circ}C$,pH 6.5, HIC 15 units and IGI 130 units. After 24h hydrolysis, more than 65% of avicel was converted to glucose and fructose; in contrast, the conversion ratio of control was 40%.

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References

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