Factors Affecting the Photooxidative Stability of Soymilk

두유의 광산화 안정성에 영향을 주는 요인

  • Published : 1996.06.01

Abstract

The effects of chlorophyll, tocopherols($\alpha$-tocopherol, ${\gamma}$-tocopherol and $\delta$-tocopherol), carotenoids ($\beta$-carotene and lutein), light sources, light intensities and strage temperatures on the photooxidative stability of soymilk were studied by measuring TBA value and depleted headspace oxygen(DHO) of soymilk. The samples were stored in the light storage box for 6 days and evaluated for the photooxidative stabilities. As the concentrations of chlorophyll increased, TBA value and DHO of the sample increased significantly(p<0.05), indicating chlorophyll acting as a photosensitizer. However, as the concentrations of tocopherols ($\alpha$-tocopherol, ${\gamma}$-tocopherol and $\delta$-tocopherol) and carotenoids ($\beta$-carotene and lutein) increased, TBA values and DHO of the samples decreased significantly(p<0.05). The light screening effects of carotenoids on DHO in the samples were not significantly different from the control at p>0.05. Therefore, there was no light screening effects of carotenoids on the oxidative stability of soymilk. The results indicate that tocopherols and carotenoids reduce the photooxidative stability of soymilk. $\delta$-Tocopherol was the most effective in photosensitized oxidation followed by ${\gamma}$-and $\alpha$-tocopherols in the order of increasing stability. $\beta$-Carotene was significantly(p<0.05) more effective than lutein in minimizing the chlorophyll-sensitized photooxidation of soymilk. Visible light was more effective than UV light in decreasing the photooxidative stability of soymilk. Therefore, photooxidation of soymilk containing chlorophyll is mainly due to photosensitized oxidation rather than photolysis reaction. As the intensities of fluorescence light increased, TBA values and DHO of the samples increased significantly at P<0.05. However, as the storage temperatures increased, TBA values and DHO of soymilk did not change significantly at p>0.05.

Keywords

References

  1. J. Am. Chem. Soc. v.61 Soymilk-New processing, packaging, expand markets Haumann, B. F.
  2. J. Food Sci. v.54 Objectional flavor of soy milk development during the soaking of soybeans and its control Matsuura, M.;Obata, A.;Fukushima, D.
  3. Prog. Lipid Res. v.23 Chemistry of free radical and singlet oxidation of lipids Frankel, E. N.
  4. Acc. Chem. Res. v.1 Photosensitized oxygenations and the role of singlet oxygen Foote, C. S.
  5. J. Am. Oil Chem. Soc. v.61 Lipid oxidation : Mechanisms, products and biological significance Frankel, E. N.
  6. CRC Crit. Rev. Food Sci. Nutr. v.10 Activated oxygen species and oxidation of food constituents Korycka-Dahl, M. B.;Richardson, T.
  7. CRC Crit. Rev. Food Sci. Nutr. v.2 Kinetics of lipid oxidation of foods Labuza, T. P.
  8. Development in dairy chemistry v.2 Lipids Richardson,T.;Korycka-Dahl, M.;Fox, P. F.(ed.)
  9. J. Food Sci. v.53 Photosensitized lipid peroxidation in ground pork and turkey Whang, K.;Peng, I. C.
  10. J. Am. Oil Chem. Soc. v.47 A possible role for singlet oxygen in the initiation of fatty acid autoxidation Rawls, H. R.;VanSanten, P. J.
  11. J. Am. Oil Chem. Soc. v.50 Participation of singlet oxygen in photosensitized oxidation of 1,4-dienoic systems and photooxidation of soybean oil Clements, A. H.;van Den Engh, R. H.;Frost, D. J.;Hoogenhout, K.;Nooi, J. R.
  12. Trends Biochem. Sci. v.2 Singlet oxygen in biological systems Krinsky, N. I.
  13. Singlet Oxygen Quenching of singlet oxygen Foote, C. S.;Wasserman, H. H.(ed.);Murray,R. W.(ed.)
  14. J. Am. Oil Chem. Soc. v.61 Prooxidant activities of chlorophylls and their decomposition products on the photooxidation of methyl linoleate Endo, Y.;Usuki, R;Kaneda, T.
  15. J. Food Sci. v.52 Effects of chlorophylls and β-carotene on the oxidative stability of olive oil Fakourelis, N.;Lee, E. C.;Min, D. B.
  16. Agric. Biol. Chem. v.48 Prooxidant activities of chlorophylls and pheophytins on the photooxidation of edible oils Usuki, R.;Endo, Y.;Kaneda, T.
  17. Lipids v.17 Photosensitized oxidation of methyl linoleate : secondary and volatile thermal decomposition products Frankel, E. N.;Neff, W. E.;Selke, E.;Weisleder, D.
  18. Lipids v.18 Photosensitized oxidation of methyl linoleate monohydroperoxides : Hydroperoxy cyclic peroxides, dihydroperoxides, keto-esters and volatile thermal decomposition products Neff, W. E.;Frankel, E. N.;Selke, E.;Weisleder, D.
  19. Lipids v.17 Photosensitized oxidation of methyl linolenate : Secondary products Neff, W. E.;Frankel, E. N.;Weisleder, D.
  20. J. Am. Chem. Soc. v.54 Products formed by photosensitized oxidation of unsaturated fatty methyl esters Terao, J.;Matsushita, S.
  21. J. Am. Chem. Soc. v.94 Singlet oxygen quenching in the liquid phase by metal chelates Carlsson, D. J.;Mendenhall, T.;Sprunchuk, T.;Wiles, D. M.
  22. Photochem. Photobiol. v.20 On the quenching of singlet oxygen by α-tocopherol Fahrenholtz, S. R.;Doleiden, F. H.;Trozollo, A. M.;Lamola, A.
  23. Photochem. Photobiol. v.20 Chemistry of singlet-oxygen. XVII. Rates of reaction and quenching of α-tocopherol and singlet oxygen Foote, C. S.;Ching, T-Y;Geller, G. G.
  24. J. Am. Chem. Soc. v.90 Chemistry of singlet oxygen quenching by β-carotene Foote, C. S.;Denny, R. W.
  25. Biochemistry v.11 Dye-sensitized photooxidation of tocopherols : Correlation between singlet oxygen reactivity and vitamin E activity Grams G. W.;Eskins, K.
  26. J. Food Sci. v.53 Quenching mechanism of β-of -carotene on the chlorophyll sensitized photooxidation of soybean oil Lee, E. C.;Min, D. B.
  27. J. Agric. Food Chem. v.38 Effects, quenching mechanisms and kinetics of carotenoids in chlorophyll-sensitized photooxidation of soybean oil Lee, S. H.;Min, D. B.
  28. J. Agric. Food Chem. v.39 Effects, quenching mechanisms, and kinetics of nickel chelates in singlet oxygen oxidation of soybean oil Lee, S. H.;Min, D. B.
  29. Frontiers of flavor Factors affecting the singlet oxygen oxidation of soybean oil Min, D. B.;Lee, E. C.;Charalambous, G.(ed.)
  30. J. Am. Oil Chem. Soc. v.64 Effects of β-carotene on light stability of soybean oil Warner, K.;Frankel, E. N.
  31. Agric. Biol. Chem. v.41 Quenching effects of tocopherols on the methyl linoleate photooxidation and their oxidation products Yamauchi, R.;Matsushita, S.
  32. J. Assoc. Off. Anal. Chem. v.66 Chloroformmethanol extraction method for determination of fat in foods: Collaborative study Daugherty, C. E.;Lento, H. G.
  33. 일본기준유지실험법 일본유지화학협회
  34. J. Am. Oil Chem. Soc. v.56 Determination of tocopherols in vegetable oils Carpenter, A. P. Jr.
  35. J. Am. Oil Chem. Soc. v.64 Adsorption of lutein from soybean oil Proctor, A.;Snyder, H. E.
  36. J. Dairy Sci. v.45 Oxidation of milk fat globule membrane material: 1. Thiobarbituric acid reaction as a measure of oxidized flavor in milk an model systems King, R. L.
  37. McGraw-Hill encyclopedia of science and technology Parker, S. P.
  38. Statistical analysis system user's guide S.A.S.