Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

The Effect of Lipid Concentration in Culture Medium on Senescence and Lipid Peroxides Production of Fibroblast from Neonate Rats

배양액내 지방함량의 변화가 신생흰쥐 피부섬유아세포의 노화와 지질과산화물 생성에 미치는 영향

  • 장영애 (이화여자대학교 식품영양학과)
  • Published : 1996.02.01
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Abstract

This study was performed to investigate the effects of lipid on cellular senescence, lipid peroxide production, and morphological changes. For this study we used primary skin fibroblasts from neonate rats grown in media various lipid contents. Fibroblasts were cultured until they lost their proliferation potential either in control medium (Dulbecco's modified Eagle's medium supplement with 10% fetal bovine serum) or in media supplemented with various concentrations of lipid-cholesterol rice component from bovine serum. Cumulative population doublings(CPD, as an index of cellular life span), and cellular thiobarbituric acid reactive substances (TBARS, as an index of lipid peroxide) concentrations were measured and morphological changes were observed. CPD were shortened with increasing lipid concentration in media ; 28.12 for cells grown in control medium and 13.42, 11.42, and 6.19 for those grown in 0.1%, 1% and 5% lipid rich components containing media, respectively. Cellular proliferation ratios were those grown in 5% lipid rich components containing media were delayed and they were degenerated soon. TBARS concentrations were increased with increasing concentration of lipid in media. Morphological changes were observed in cells grown in control medium by cellular senescence. Especially lipid droplets were observed in cells grown in 5% lipid rich components containing media. Therefore it seems that lipid contents in media had an effect on cellular proliferation and cellular life span, possibly via lipid peroxide production.

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References

  1. Exp Cell Res v.25 The serial cultivation of human diploid cell strains Hayflick,L.;Moorhead,P.S.
  2. Ann Ny Acad Sci v.663 Molecular changed with in vitro cellular senescence Cristofalo,V.J.;Pignolo,R.J.;Rotenberg,M.O.
  3. Growth control during cell aging Inhibitiotors of DNA synthesis in senescent and quiescent human diploid fibroblasts Gretchen,H.S.;Wang,E.(ed.);Warner,H.R.(ed.)
  4. Proc Natl Acad Sci USA v.85 Human skin fibroblasts in vitro differentiation along a terminal cell lineage Bayreuther,K.;Rodemann,H.P.;Hommel,R.;Dittmann Kalbeiz M.;Francz,P.I.
  5. 한국영양학회지 v.24 no.5 식이지방이 나이에 따른 흰쥐 섬유아세포의 노화과정 및 면역반응에 미치는 영향 조미숙;김화영
  6. J Nutr v.51 The influence of dietary fat and carbohydrate on growth and longevity in rats French,G.R.;Ingram,R.H.;Uram,A.J.;Barrow,G.P.;Surf,R.W.
  7. Lipid peroxides in biology and medicine Assay for serum lipid peroxide level and its clinical significance Yagi,K.;Yagi,K.(ed.)
  8. Toxicol Industrial Health v.9 Overview, mechanisms of antioxidant action on life span Waner,H.R.
  9. Lipids v.15 no.6 Utilization of polyunsaturated fatty acids by human diploid cells aging in vitro Lynch,R.D.
  10. Am J Clin Nutr v.53 Membrane damage due to lipid peroxidation NiKi,E.;Yamamoto,Y.;Komura,E.;Sato,K.
  11. Animal cell culture Aging of cultured Human skin fibroblasts Harley,C.B.;Pollard,J.W.(ed.);Waker,J.M.(ed.)
  12. Cell growth and division Cell culture of human diploid fibroblasts in serum-containing medium and serum-free chemically defined medium Cristofalo,V.J.;Phillips,P.D.;Baserga,R.(ed.)
  13. Cell growth and division Measuring parameters of growth Baserga,R.;Baserga,R.(ed.)
  14. Methods in enzymology Assay for blood plasma or serum Yagi,K.
  15. Growth control during cell aging Finite proliferative capacity of Syrian hamster fetal and adult fibroblasts in vitro: A model system for the analysis of the cellular basis of aging Bruce,S.A.;Wang,E.(ed.);Wanner,H.R.(ed.)
  16. Ann NY Acad Sci v.663 Lipid peroxidation and hepatocyte death investigation of a possible mechanism of oxidative cell injury Carini,R.;Parola,M.;Dianzani,M.U.;Albano,E.
  17. Exp Gerontol v.26 Changes in enzyme activities in skin fibroblasts derived from persons of various ages Shindo,Y.;.Akiyama,J.;Yamazaki,Y.;Saito,K.;Takase,Y.
  18. Am J Clin Nutr v.53 Membrane damage due to lipid peroxidation NiKi,E.;Yamamoto,Y.;Komura,E.;Sato,K.
  19. Mech Age Dev v.14 Recent advances in the cell biology of aging Hayflick,L.
  20. Biochim Biophysia Acta v.838 Subcellular localization and modification with aging of glutathione, glutathione peroxidase and glutathione reductase activities in human fibroblasts Mbemba,F.;Houbion,A.;Raes,M.;Remacle,J.
  21. Ann NY Acad Sci v.663 Cell proliferation and cell death in immunosenescence Monti,D.;Troland,L.;Grassilli,E.;Agnesini,C.;Tropea,F.;Barbieri,D.;Capri,M.;Cristofalo,E.A.;Salvioli,S.;Ronchetti,I.;Bellomo,G.;Cossarizza,A.;Franceschi,C.
  22. Proc Natl Acad Sci USA v.82 Peroxide-producing potential of tissues: Inverse correlation with longevity of mammalian species Cutler,R.G.
  23. 노화 김숙희;김화영
  24. J Gerontol v.33 no.2 Body Weight increment and length of life: The effect of genetic constitution and dietary protein Goodlich,C.L.
  25. Ann NY Acad Sci v.663 Oxidants are major contributor to aging Ames,B.N.;Shigenaga,M.K.