Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Comparative studies of the five edible mountain vegetables by DNA marker fingerprinting

DNA marker 지문법에 의한 취나물 5종 (청옥취 , 개미취 , 참취 , 수리취 , 곰취)의 비교연구

  • Published : 1996.12.01
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Abstract

Five edible mountain vegetables(Saussurea sp. Aster tataricus, A. scaber. Synurus deltoides, Ligularia fischeri) were investigated on the basis of amplified DNA polymorphisms resulted from PCR (polymerase chain reaction) analysis. The sampled plants consisted of 38 individuals in 5 taxa. Only 10 primers out of 62 primers (60 random [10-mer] primers, two 15-mer [M13 core sequence, and (GGAT) sequence]) tested gave rise to polymorphisms in all of the tested plants, producing 176 DAN fragments amplified. Intraspecific polymorphisms found in each taxa showed intraspecies constancy (31.1-61.1%) in the banding patterns of individual plants: Saussurea sp. 31.1%, 15 bands, Aster tataricus, 40.9%, 18 bands, A. scaber. 38.5%, 15 bands. Synurus deltoides, 34.7%, 17 bands, and Ligularia fischeri, 61.1%, 22 brands, respectively. All five species were well classified from each other at the 0.93 level of similarity index value. Intraspecific and interspecific variations were appeared at the levels ranging from 0.62 to 0.99. Based on these results, our PCR analyses support the previous data derived from external morphology of the 5 edible mountain vegetables, but very low levels o intraspecific variations were detected in all of these taxa.

유용 취나물 5종류의 종간 유사도 및 종내 변이를 파악하기 위하여 38개체를 대상으로 PCR 방법을 이용하여 비교분석하였다. 사용된 62가지 primer 중에서 38개체 전체에서 band를 보여준 것은 10가지였고, 이를 통해 얻어진 총 band의 수는 176개로 나타났다. 종내변이는 청옥취가 31.1%에 해당하는 15개의 polymorphic band를 가져 변이가 가장 낮았으며 곰취는 61.1%로 가장 높게 나타났다. 군집분석 결과 조사된 38개체는 유사도 0.93에서 5종의 구분이 가능하였으며 38개체 각각은 유사도 0.62-0.99의 유사성을 갖는 것으로 나타났고 종내변이는 0.93이상으로 높게 나타나 변이가 적은 것으로 나타났다. 이상의 결과에서 PCR방법을 이용한 결과가 취나물 5종류를 구분하는데 유용하게 사용되었으며 군집분석 결과 종내 변이가 매우 낮은 것으로 나타났다.

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