Effect of pH on the Production of Lactic Acid and Secondary Products in Batch Cultures of Lactobacillus casei

  • Yoo, Ik-Keun (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Chang, Ho-Nam (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Lee, Eun-Gyo (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Chang, Yong-Keun (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Moon, Seung-Hyeon (Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology)
  • Published : 1996.12.01

Abstract

Batch fermentations of lactic acid were performed with Lactobacillus casei to investigate the effect of pH on cell growth and production of lactic acid and by-products. Maximum productivity of lactic acid increased with increasing pH from 5.0 to 6.5, and the extent of D-lactate production was different at each pH. Acetate and D-lactate concentrations increased even after the complete consumption of glucose in the medium. While a pH range of 6.0-6.5 was optimal for cell growth and lactic acid production, superior results were achieved at pH 6.0 when both maximum lactic acid productivity and minimum by-product formation were considered.

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