Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Analysis of Genetic Polymorphism Among Six Korean Wild Artemisia spp. by Using RAPD Method

RAPD 방법을 이용한 한국 야생쑥 6종간의 유전적 유연관계 분석

  • Pyo, Hyun Jin (Dept. of Agricultural Biology, Chungnam National University) ;
  • Choi, Kwan Sam (Dept. of Agricultural Biology, Chungnam National University)
  • 표현진 (충남대학교 농과대학 농생물학과) ;
  • 최관삼 (충남대학교 농과대학 농생물학과)
  • Published : 1996.06.30
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Abstract

Eighteen nuclear probes were used to examine RFLP(restriction fragment length polymorphism) between six species of Artemisia spp. of Korea. Total DNA from six different species of Artemisia was separately cut with three restrict enzymes. The PstI enzyme was showed to reduce the variation of polymorphisms than the other two enzymes(EcoRl and BamHI). The genetic variation of polymorphism was similar between the Dhewegiki-ssug and Cham-ssug. RAPD analysis was applied to the same six species of Artemisia spp. in order to assess the degree of DNA polymorphism within the Artemisia genus. Six species of Artemisia were evaluated for variation using a set of 11 random 10-mer primers. Nine out of the eleven primers revealed scorable polymorphisms between six species of Artemisia spp. Genetic distances between each of the species were calculated and cluster analysis was used to generate a dendrogram showing phylogenetic relationships between them This result indicates that molecular markers will be more usable in intraspecific study of Artemisia spp. than isoenzyme markers.

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