Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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DNA fingerprinting patterns of 5 serotypes of Actinobacillus actinomycetemcomitans

Actinobacillus actinomycetemcomitans의 혈청형별 제한절편장 다변화에 관한 연구

  • Choi, Jeom-Il (Departments of Periodontology, School of Dentistry, Pusan National University) ;
  • Koh, Myung-Yun (Departments of Oral Medicine, School of Dentistry, Pusan National University) ;
  • Yun, Il (Departments of Dental Pharmacology and Biophysics, School of Dentistry, Pusan National University)
  • 최점일 (부산대학교 치과대학 치주과) ;
  • 고명연 (부산대학교 치과대학 구강내과) ;
  • 윤일 (부산대학교 치과대학 치과약리학교실)
  • Published : 1996.06.30
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Abstract

5 serotypes(a, b, c, d, e) of Actinobacillus actinomycetemcomitans showed distinct hybridization patterns(DNA fingerprinting patterns) when the bacterial DNA were hybridized with randomly cloned 4.7-Kb sized DNA probe. The sizes of hybridized bands in each serotypes were different among serotypes and represented unique patterns of hybridization with the probe used. The serotype a showed two bands of fingerprinting patterns: 23.1 kb and 2.5 kb respectively. Serotype b and c showed single band: 6.6 kb and 9.5 kb, respectively. Serotype d and e showed two bands of hybridization: 23.1 kb and 2.8 kb, and 23.7 kb and 2.1 kb, respectively. The results indicate that this standard fingerpriting patterns of DNA hybridization with 4.7 kb probe can be further used for genotyping clinical isolates of Actinobacillus 8ctinomycetemcomitansand its relevance with periodontal disease activity.

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