Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

HEMAGGLUTINATION AND COLONY HYBRIDIZATION FOR THE IDENTIFICATION OF ENTEROTOXIGENIC Escherichia coli ISOLATED FROM HEALTHY PIG

  • Choi, S.H. (Department of Food Science and Technology, Chungnam National University) ;
  • Oh, M.J. (Department of Food Science and Technology, Chungnam National University) ;
  • Sung, C. (Department of Food Science and Technology, College of Agriculture, Chungnam National University)
  • Received : 1996.03.04
  • Accepted : 1996.07.01
  • Published : 1996.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Erythrocytes from three different animal species were used to determine mannose-sensitive hemagglutination (MSHA) and mannose-resistant hemagglutination (MRHA) of 755 isolates obtained from rectal swabs of healthy pig. In addition, colony hybridization using digoxigenin-dUTP labeled polynucleotide probes was performed for the detection of heat-stable and heat-labile enterotoxin genes carried by MRHA positive isolates. Of 755 strains, 9, 4 and 28 strains gave a positive MRHA with bovine, equine and pig erythrocytes, respectively. Of these isolates, 28 (3.7%) were characterized for positive MRHA by at least one blood. Seven isolates gave a positive MRHA with two kinds of blood. Three gave a positive MRHA with three kinds of blood. Twenty-eight strains, while positive in MRHA, yielded negative signals in the colony hybridization assay for the detection of heat-stable (STaI and STaII) and heat-labile (LT) enterotoxin genes in E. coli.

Keywords