Immunohistochemical Study on the Inhibition of T lymphocytic Differentiation and Secretion of IL-2 in Mouse Thymus by Chronic Alcohol administration

장기간 알콜 투여가 생쥐 가슴샘에서 T 림프구의 분화와 IL-2 분비 저해에 미치는 면역조직화학적 연구

  • Kim, Jin Taek (Dept. of Anatomy, Oriental Medical College, Dongguk University) ;
  • Park, In Sick (Dept. of Anatomy, Oriental Medical College, Dongguk University) ;
  • Ahn, Sang Hyun (Dept. of Anatomy, Oriental Medical College, Dongguk University)
  • 김진택 (동국대학교 한의과대학 해부학교실) ;
  • 박인식 (동국대학교 한의과대학 해부학교실) ;
  • 안상현 (동국대학교 한의과대학 해부학교실)
  • Published : 1996.12.20

Abstract

Alcohol is a major risk factor for several diseases and especially excessive, long-term alcohol consumption are caues the damage of immunity such as the inhibiton of secretion of lymphokine and proliferation of immune component cell. This study observed that the inhibition of T lymphocytic differentiation and secretion of interleukin 2(IL-2) induced in thymus of ICR mouse by chronic alcohol administration. After 8% alcohol voluntary administered for 120 days, the thymic tissue immunohistochemically stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly-2(CD8), and IL-2 receptor(CD25R) after embedding with paraffin. The results were as follows. 1. The size of thymic medulla in test group reduced than control group. 2. The number of helper T lymphocyte, cytotoxic T lymphocyte, and IL-2 receptor were decreased in thymic medulla and cortico-medullary junction of test group and the degree of CD4, CD8, and CD25R positive reaction were soften in test group. These results indicated that the secretion of IL-2 in thymus was inhibited by chronic alcohol administration and subsequently prevent to differentiate from thymocytes to T lymphocytes. As this view, cell mediated immunity were reduced by chronic alcohol administration.

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