BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Increase in Linolenate Contents by Expression of the fad3 Gene in Transgenic Tobacco Plants

  • Kang, Young-Hwi (Department of Agricultural Chemistry, Korea University) ;
  • Min, Bok-Kee (Department of Agricultural Chemistry, Korea University) ;
  • Park, Hee-Sung (Department of Plant Breeding, Catholic University of Taegu-Hyosung) ;
  • Lim, Kyung-Jun (Department of Genetic Engineering, Kyungpook National University) ;
  • Huh, Tae-Lin (Department of Genetic Engineering, Kyungpook National University) ;
  • Lee, Se-Yong (Department of Agricultural Chemistry, Korea University)
  • Received : 1996.02.22
  • Published : 1996.07.31
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Abstract

An 1.4 kb of the fad3 cDNA encoding microsomal linoleic acid desaturase catalyzing the conversion of linoleic acid (18:2, ${\omega}-6$) to linolenic acid (18:2, ${\omega}-3$) was introduced into tobacco plants by the Agrobacterium-mediated plant transformation, Among the transgenic tobacco plants conferring kanamycin resistance, five transformants showing increment in unsaturated fatty acid contents were selected and further analyzed for the transgenecity, In genomic Southern blot analyses, copy numbers of the integrated fad3 DNA in chromosomal DNA of the five transgenic tobacco plants were varied among the transgenic lines. By Northern blot analyses, the abundancy of the fad3 mRNA transcript directed by Cauliflower Mosaic Virus 35S promoter was consistent with the relative copy number of the fad3 DNA integrated in the chromosome of transgenic tobacco plants. When compared with the wild type, accumulation of linolenic acid in transgenic tobacco roots was elevated 3.7- to 4.7-fold showing a corresponding decrease in the linoleic acid contents; however, slight increments for linolenic acid were noticed in transgenic leaf tissues. These results indicated that the elevated level of fad3 expression is achieved in transgenic tobacco plants.

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References

  1. Science v.258 Arondel, V.;Lemieux, B.;Hwang, I.;Gibson, S.;Goodman, H.M.;Somerville, C.R. https://doi.org/10.1126/science.1455229
  2. Ann. Rev. Plant Physiol. Plant Mol. Biol. v.42 Browse, J.;Somerville, C.R. https://doi.org/10.1146/annurev.pp.42.060191.002343
  3. Lipids v.25 Chee, K.M.;Gong, J.X.;Meydani, M.;Ausman, L.;Johnson, J.;Siguel, E.N.;Schaefer, E.F. https://doi.org/10.1007/BF02537158
  4. Anal. Biochem. v.162 Chomezynsky, P.;Sacchi, N.
  5. Plant Mol. Biol. Reporter v.9 Gawel, N.J.;Jarret, R.L. https://doi.org/10.1007/BF02672076
  6. Plant Physiol. v.41 Gerloff, E.D.;Richardson, T.;Stahmann, M.A. https://doi.org/10.1104/pp.41.8.1280
  7. Ann. Rev. Plant Physiol. v.33 Graham, D.;Patterson, B.D. https://doi.org/10.1146/annurev.pp.33.060182.002023
  8. Mol. Gen. Genet. v.163 Holster, M.;de Waele, D.;Depicker, A.;Messens, E.;Van Montegue, M.;Schell, J. https://doi.org/10.1007/BF00267408
  9. Science v.277 Horsch, R.B.;Fry, J.E.;Hoffman, N.L.;Eichnoltz, S.;Rogers, ？S.C.;Farely, R.T.
  10. Plant Physiol. v.99 Hugly, S.;Somerville, C.R. https://doi.org/10.1104/pp.99.1.197
  11. J. Biol. Chem. v.268 Iba, K.;Gibson, S.;Nishiuchi, T.;Fuse, T.;Nishimura, M.;Arondel, V.;Hugly, S.;Somerville, C.R.
  12. Plant Physiol. v.105 Kodama, J.;Hamada, T.;Horiguchi, G.;Nishimura, M.;Iba, K. https://doi.org/10.1104/pp.105.2.601
  13. Plant Physiol. v.39 Lyons. J.M.;Wheaton, T.A.;Pratt, H. https://doi.org/10.1104/pp.39.2.262
  14. J. Bacteriol. v.84 Marr, A.G.;Ingraham, J.L.
  15. Proc. Natl. Acad. Sci. USA v.90 Miquel, M.;James, D.J.;Dooner, H. Jr.;Browse, J. https://doi.org/10.1073/pnas.90.13.6208
  16. Physiol. Plant v.15 Murashige, T.;Skoog, F. https://doi.org/10.1111/j.1399-3054.1962.tb08047.x
  17. Molecular Cloning, A Laboratory Manual Sambrook, J.;Fritsch, E.F.;Maniatis, T.
  18. Science v.252 Somerville, C.R.;Browse, J. https://doi.org/10.1126/science.252.5002.80
  19. Crop Sci. v.19 St. John, J.B.;Christiansen, M.N.;Ashworth, E.N.;Gentmer, W.A. https://doi.org/10.2135/cropsci1979.0011183X001900010015x
  20. Plant Physiol. v.103 Yadav, N.S.;Wierzbicki, A.;Aegerter, M.;Caster, C.S.;Perez-Grau, L.;Kinney, A.J.;Hitz, W.D.;Booth, J.R.B.;Schweiger, B. Jr.;Stecca, K.L.;Allen, S.M.;Blackwell, M.;Reiter, R.S.;Carlson, T.J.;Russel, S.H.;Feldmann, K.A.;Pierce, J.;Browse, J. https://doi.org/10.1104/pp.103.2.467